Anti-STUB1 Antibody (monoclonal, 13I8)价格_品牌:BOSTER-丁香通官网

克隆性：Monoclonal(Clone:13I8)

适应物种：human, monkey, mouse, rat

保质期：发货周期：现货

库存：9999

供应商：博士德生物

应用范围：WB, IHC, FCM

规格：50μl/100μl/150μl

产品概况

货号	M01236
产品名称	Anti-STUB1 Antibody (monoclonal, 13I8)
基因名	STUB1
抗体来源	Mouse
克隆	Monoclonal(Clone:13I8)
抗体亚型	Mouse IgG2b
分子量	35KD
免疫原	E.coli-derived human STUB1 recombinant protein (Position: R51-Y303).
内容	200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式	protein G purified.
浓度	200ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	STUB1 (STIP1 homology and U-Box containing protein 1), also known as CHIP (C terminus of HSC70-Interacting Protein), is a human gene. This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2.
Uniprot ID	STUB1: Q9UNE7
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry in paraffin section (IHC):	1:50-400
Flow cytometry (FCM):	1-3 μg/1x10⁶ cells
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- STUB1 antibody (M01236).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates.
Lane 4: human THP-1 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human U87 whole cell lysates,
Lane 7: rat heart tissue lysates,
Lane 8: rat brain tissue lysates,
Lane 9: mouse brain tissue lysates,

Lane 10: mouse HEPA1-6 whole cell lysates.Use rabbit anti- STUB1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for STUB1 at approximately 35KD. The expected band size for STUB1 is at 35KD.|Figure 2.IHC analysis using anti- STUB1 antibody (M01236). detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3.IHC analysis using anti- STUB1 antibody (M01236). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4.IHC analysis using anti- STUB1 antibody (M01236). detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5.IHC analysis using anti- STUB1 antibody (M01236). detected in paraffin-embedded section of mouse small intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6.IHC analysis using anti- STUB1 antibody (M01236). detected in paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 7.Flow cytometry analysis of A549 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]