Anti-SOX15 Antibody价格_品牌:BOSTER-丁香通官网

克隆性：Polyclonal

适应物种：human, mouse, rat

保质期：发货周期：现货

库存：9999

供应商：博士德生物

应用范围：WB, IHC, ICC/IF, ELISA

规格：50μl/100μl/150μl

产品概况

货号	A07722-1
产品名称	Anti-SOX15 Antibody
基因名	SOX15
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	26KD
免疫原	E.coli-derived human SOX15 recombinant protein (Position: M1-L233).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Protein SOX-15 is a protein that in humans is encoded by the SOX15 gene. This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins.
研究类别	1. Cremazy, F., Soullier, S., Berta, P., Jay, P. Further complexity of the human SOX gene family revealed by the combined use of highly degenerate primers and nested PCR. FEBS Lett. 438: 311-314, 1998.2. Lee, H.-J., Goring, W., Ochs, M., Muhlfeld, C., Steding, G., Paprotta, I., Engel, W., Adham, I. M.Sox15 is required for skeletal muscle regeneration. Molec. Cell. Biol. 24: 8428-8436, 2004.
Uniprot ID	SOX15: O60248
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry in paraffin section (IHC):	1:50-400
Immunocytochemistry in fixed cells:	1:50-400
(ELISA)：	1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of SOX15 using anti-SOX15 antibody (A07722-1). Lane 1: human A431 whole cell lysatesLane 2: human U-87MG whole cell lysatesLane 3: human HEK293 whole cell lysatesLane 4: human K562 whole cell lysatesLane 5: human U2OS whole cell lysatesLane 6: rat thymus tissue lysatesLane 7: mouse SP20 whole cell lysates anti-SOX15 antigen affinity purified polyclonal antibody (Catalog # A07722-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SOX15 at approximately 29KD. The expected band size for SOX15 is at 26KD.|Figure 2. IF analysis of SOX15 using anti- SOX15 antibody (A07722-1).
SOX15 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 3. IHC analysis of SOX15 using anti-SOX15 antibody (A07722-1).SOX15 was detected in paraffin-embedded section of mouse brain tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of SOX15 using anti-SOX15 antibody (A07722-1).SOX15 was detected in paraffin-embedded section of rat testis tissues.Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of SOX15 using anti-SOX15 antibody (A07722-1).SOX15 was detected in paraffin-embedded section of human testis cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of SOX15 using anti-SOX15 antibody (A07722-1).SOX15 was detected in paraffin-embedded section of mouse testis tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. [/list_product_images]