Anti-p70 S6 Kinase (Ab-411) RPS6KB1 Antibody

价格： ￥3588

品牌：BOSTER 品牌认证

货号：A01475-1

产品详情

产品概况

货号	A01475-1
产品名称	Anti-p70 S6 Kinase (Ab-411) RPS6KB1 Antibody
基因名	RPS6KB1
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit
分子量	59KD
免疫原	Peptide sequence around aa. 409~413 (I-R-S-P-R) derived from Human p70 S6 Kinase.
内容	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
纯化方式	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
浓度	500 ug/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Uniprot ID	RPS6KB1: P23443

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blotting:	1:500~1:1000
Immunohistochemistry:	1:50~1:100
Immunofluorescence (IF):	1:100~1:200

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

点击图片放大

[list_product_images]Figure 1. Western blot analysis of RPS6KB1 using anti-RPS6KB1 antibody (A01475-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KB1 antigen affinity purified polyclonal antibody (Catalog # A01475-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for RPS6KB1. | Figure 2. IHC analysis of RPS6KB1 using anti-RPS6KB1 antibody (A01475-1). RPS6KB1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RPS6KB1 Antibody (A01475-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. | Figure 3. Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase(Ab-411) Antibody #A01475-1.[/list_product_images]