Anti-Neurofilament NF-H/NEFH Antibody

价格： ￥3588

品牌：BOSTER 品牌认证

货号：M05307-6

产品详情

产品概况

货号	M05307-6
产品名称	Anti-Neurofilament NF-H/NEFH Antibody
基因名	NEFH
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit
分子量	117-220KD
免疫原	Native NF-H purified from bovine spinal cord
内容	Antibody is supplied as an aliquot of serum
浓度	500 ug/ml
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Uniprot ID	NEFH: P12036

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

WB: 1:10,000-25,000. ICC/IF and IHC: 1:1,000-5,000.

应用	稀释度*

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Western blot analysis of different tissue lysates using rabbit pAb to NF-H, M05307-6, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected with M05307-6 antibody.|Immunohistological analysis of a mouse hippocampus section stained with rabbit pAb to NF-H, M05307-6, dilution 1:2,000 in red, and costained with mouse mAb to myelin basic protein (MBP), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45?M, and free-floating sections were stained with above antibodies. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.[/list_product_images]