Anti-Ki67 Monoclonal Antibody

价格： ￥3588

品牌：BOSTER 品牌认证

货号：M00254-2

产品详情

产品概况

货号	M00254-2
产品名称	Anti-Ki67 monoclonal Antibody
基因名	MKI67
抗体来源	Mouse
克隆	Monoclonal
抗体亚型	Mouse
分子量	359KD
免疫原	Recombinant construct containing the 2nd, 3rd and 4th Ki67 repeats of the human sequence (amino acids 1,111-1,490) expressed in and purified from E. coli.
内容	Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol, 5mM azide
浓度	500 ug/ml
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Uniprot ID	MKI67: P46013
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

WB: 1:1,000-5,000. IF 1:2,000-5,000

应用	稀释度*

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Western blot analysis of equal amounts of cell lysates using mouse mAb to Ki67, M00254-2, dilution 1:2,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of apparent molecular weight of 345kDa and 395kDa. Smaller proteolytic fragments of these isoforms are also invariably detected on the blot.|High magnification confocal immunofluorescence image of M00254-2 stained HeLa cells grown in tissue culture, in green. The nuclei of rapidly dividing cells have clumps of Ki-67 associated with nucleoli, while nearby quiescent cells express little or no Ki-67. Cells are counterstained with other in red which reveals the nuclear lamina, and DAPI reveals nuclear DNA in blue.[/list_product_images]