Anti-NeuN Antibody

价格： ￥3588

品牌：BOSTER 品牌认证

货号：M11954-1

产品详情

产品概况

货号	M11954-1
产品名称	Anti-NeuN Antibody
基因名	RBFOX3
抗体来源	Mouse
克隆	Monoclonal
抗体亚型	Mouse
分子量	46-55KD
免疫原	N-terminal 99 amino acids of human FOX3 expressed in and purified from E. coli
内容	Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃
浓度	500 ug/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Uniprot ID	RBFOX3: A6NFN3
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:1000-2000
Immunohistochemistry in paraffin section (IHC):	1:500-1000
Immunofluorescence (IF):	1:50-1000
Immunocytochemistry in fixed cells(ICC):	1:50-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Western blot analysis of whole brain tissue lysates using mouse mAb to FOX3/NeuN M11954-1, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Note the strong twin bands corresponding to the two alternate transcripts of FOX3/NeuN protein with apparent SDS-PAGE molecular weights of 46 and 48kDa. As with other FOX3/NeuN antibodies, an additional band at ~70kDa is revealed in some lysates.|Immunofluorescent analysis of rat brain stem costained with mouse mAb to FOX3/NeuN M11954-1 in green, and chicken pAb to microtubule associated protein 2 (MAP2) in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45?M, and free-floating sections were stained with the above antibodies. The Fox3/NeuN antibody selectively stains nuclei and the proximal cytoplasm of neuronal cells while the MAP2 antibody labels dendrites and overlaps with Fox3/NeuN staining in the perikarya of neurons.[/list_product_images]