Anti-MRC1 Antibody价格_品牌:BOSTER-丁香通官网

克隆性：Polyclonal

适应物种：human, mouse, monkey, rat

保质期：发货周期：现货

库存：9999

供应商：博士德生物

应用范围：WB, IHC, Direct ELISA

规格：50μl/100μl/150μl

产品概况

货号	A02285-2
产品名称	Anti-MRC1 Antibody
基因名	MRC1
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	190-200KD
免疫原	E.coli-derived human Mannose Receptor/MRC1 recombinant protein (Position: D21-A1140).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing.
背景资料	The mannose receptor (Cluster of Differentiation 206,CD206) is a C-type lectin primarily present on the surface of macrophages,immature dendritic cells and liver sinusoidal endothelial cells,but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is mapped to 10p12.33. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes,including cell-cell recognition,serum glycoprotein turnover,and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses,bacteria,and fungi so that they can be neutralized by phagocytic engulfment.
Uniprot ID	MRC1: P22897
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:500-2000
Immunohistochemistry in paraffin section (IHC):	1:50-400
Direct ELISA：	1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti-MRC1 antibody (A02285-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: human liver tissue lysates,
Lane 4: monkey lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MRC1 antigen affinity purified polyclonal antibody (A02285-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MRC1 at approximately 190-200 kDa. The expected band size for MRC1 is at 166 kDa.|Figure 2. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 4. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 5. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.[/list_product_images]