Anti-MCAM Antibody(monoclonal, 2H12)价格_品牌:BOSTER-丁香通官网

克隆性：Monoclonal(Clone:2H12)

适应物种：human

保质期：发货周期：现货

库存：9999

供应商：博士德生物

应用范围：IHC, IHC-F, WB

规格：50μl/100μl/150μl

产品概况

货号	M01683-4
产品名称	Anti-MCAM Antibody(monoclonal, 2H12)
基因名	MCAM
抗体来源	Mouse
克隆	Monoclonal(Clone:2H12)
抗体亚型	Mouse IgG1
分子量	120KD
免疫原	E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	protein G purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.
研究类别	1. Bardin, N., Anfosso, F., Masse, J.-M., Cramer, E., Sabatier, F., Le Bivic, A., Sampol, J., Dignat-George, F.Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion.Blood 98: 3677-3684, 2001.2. Kuske, M. D. A., Johnson, J. P.Assignment of the human melanoma cell adhesion molecule gene (MCAM) to chromosome 11 band q23.3 by radiation hybrid mapping.Cytogenet. Cell Genet. 87: 258 only, 1999. 3. Taira, E., Nagino, T., Taniura, H., Takaha, N., Kim, C.-H., Kuo, C.-H., Li, B.-S., Higuchi, H., Miki, N.Expression and functional analysis of a novel isoform of gicerin, an immunoglobulin superfamily cell adhesion molecule.J. Biol. Chem. 270: 28681-28687, 1995.
Uniprot ID	MCAM: P43121
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry(Paraffin-embedded Section):	1:50-400
Immunohistochemistry(Frozen Section):	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- MCAM antibody (M01683-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human HELA whole cell lysates.Use rabbit anti- MCAM 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for MCAM at approximately 120KD. The expected band size for MCAM is at 120KD.|Figure 2.IHC analysis using anti- MCAM antibody (M01683-4). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3.IHC analysis using anti- MCAM antibody (M01683-4). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.[/list_product_images]