Human CD3e / CD3 epsilon CHO Cell Lysate (WB positive control)

免疫原 ：A DNA sequence encoding the human CD3E (NP_000724.1) (Met1-Asp126) was expressed with the Fc region of human IgG1 at the C-terminus.

亚型 ：见说明书

形态 ：液体

保存条件 ：4℃

克隆性 ：无

标记物 ：见说明书

适应物种 ：Human

保质期 ：12个月

抗原来源 ：见说明书

目录编号 ：10977-H02HL

级别 ：免疫学

库存 ：99

供应商 ：北京义翘神州科技股份有限公司

宿主 ：HEK293 Cells

应用范围 ：WB

浓度 ：见说明书

靶点 ：CD3E

抗体英文名 ：Human CD3e / CD3 epsilon CHO Cell Lysate (WB positive control)

抗体名 ：Human CD3e / CD3 epsilon CHO Cell Lysate (WB positive control)

规格 ：300 µg

Human CD3e / CD3 epsilon CHO Cell Lysate (WB positive control)（产品说明）
反应种属：Human
裂解液靶点：CD3E
裂解液应用：WB
裂解液保存条件：Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述：HEK293 Cell Human CD3e / CD3 epsilon transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主：HEK293 Cells
裂解液制备方法：Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer：Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息：12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议：1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
裂解液稳定性：Samples are stable for up to twelve months from date of receipt.
裂解液使用说明：Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested.