英文名 ：Recombinant 2019-nCoV S Protein RBD-SD1 (C-6His)

保存条件 ：Reconstituted protein solution should be stored at ≤ -20°C.

规格 ：50μg/500μg/1mg

Recombinant 2019-nCoV S Protein RBD-SD1 (C-6His)

 

Catalog #:  DRA42       Derived From: Human Cells  

 

Description:

Recombinant 2019-nCoV S Protein RBD-SD1 is produced by our Mammalian expression system and the target gene encoding Arg319-Ser591 is expressed with a 6His tag at the C-terminus.

Accession:  QHD43416.1

Names(Known as):  2019-nCov RBD Protein; 2019-nCoV Spike RBD Protein

 

Quality Control:

Mol Mass:  31.4kDa      AP Mol Mass:  35-43kDa, reducing conditions

Purity:  Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity: 

Immobilized Human ACE-2-Fc(Cat#C05Y) at 10μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-SD1-His(Cat#DRA42).The ED50 of Recombinant 2019-nCoV S Protein RBD-SD1-His(Cat#DRA42) is 0.64 ug/ml.

 

Formulation:

Supplied as a 0.2 μM filtered solution of PBS,PH7.4.

 

Shipping:

The product is shipped on dry ice pack.

Upon receipt, store it immediately at the temperature listed below.

 

Storage:

Reconstituted protein solution should be stored at ≤ -20°C.

 

Purification: 
Affinity purification chromatography.

 

Immunoreactivity: 
N.A.

 

Application:
Immunogen, calibrator or standard.

 

Background:

Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY