G.SS Rabbit Polyclonal Antibody (KO Validated)

规格 ：50μl

产品编号	产品名称	产品包装	产品价格
AF5176	G.SS Rabbit Polyclonal Antibody (KO Validated)	50μl	593.00元

来源	用途	交叉反应性	理论分子量	实际分子量
Rabbit	WB	H, M, R	40/52kDa	54kDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB	IP	IF	IHC	ICC	FC	ChIP	ChIP-seq
1:500-1:2000	-	-	-	-	-	-	-

抗体详细信息如下:

About this Antibody
Name	G.SS Rabbit Polyclonal Antibody (KO Validated)
Category	Rabbit Polyclonal Antibody (pAb); Primary antibody
Isotype	IgG
Purification method	Af.finity purification
Positive samples	DU145, K-562, 293T, Mouse kidney, Mouse spleen, Mouse brain, Rat kidney
Cellular location	-
Customer validation	-
About the Immunogen
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human G.SS (NP_000169.1).
Sequence	NLYGEEMVQALKQLKDSEERASYILMEKIEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV
Gene ID	2937
Swiss Prot	P48637
Synonyms	G.SS; GSHS; HEL-S-64p; HEL-S-88n; glutathione synthetase
Category	Glutamine Metabolism
Background	G.SS (Glutathione synthetase) is a 474 amino acid protein encoded by the gene located at human chromosome 20q11.2. GS.S consists of three loops projecting from an antiparallel β-sheet, a parallel β-sheet and a lid of anti-parallel sheets, which provide access to the ATP-binding site. Although Southern blot and gene analysis suggest that G.SS may be the only member of a unique family, the crystal structure indicates that G.SS belongs to the ATP-GRASP superfamily. GS.S is expressed in hemocytes and nucleated cells, including the brain. G.SS occurs as a homodimer. There are two steps in the production of Glutathione, begining with γ-GCS and ending with GS.S. In an ATP-dependent reaction, GS.S produces Glutathione from γ-glutamylcysteine and glycine precursors. Partial hepatectomy, diethyl maleate, buthionine sulfoximine, tert-butylhaydroquinone and thioacetamid.e increase the ex-pression of GS.S, which causes an increase in Glutathione levels. An inherited autosomal recessive disorder, 5-oxoprolinuria (pyroglutamic aciduria), is caused by GS.S deficiencies, which leads to central nervous system damage, hemolytic anemia, metabolic acidosis and urinary excretion of 5-oxoproline. A missense mutation in the gene encoding GS.S leads to a G.SS deficiency restricted to erythrocytes, which causes only hemolytic anemia.

包装清单：

产品编号	产品名称	包装
AF5176	G.SS Rabbit Polyclonal Antibody (KO Validated)	50μl
AZ050	Western一抗稀释液	50ml
—	说明书	1份

保存条件：
G.SS Rabbit Polyclonal Antibody (KO Validated) -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。