N6-Methyladenosine (m6A) Rabbit Polyclonal Antibody

供应商 ：上海信裕生物科技有限公司

库存 ：50

克隆性 ：多克隆

抗原来源 ：Rabbit

保质期 ：一年

抗体英文名 ：N6-Methyladenosine (m6A) Rabbit Polyclonal Antibody

抗体名 ：N6-Methyladenosine (m6A) Rabbit Polyclonal Antibody

保存条件 ：N6-Methyladenosine (m6A) Rabbit Polyclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。

N6-Methyladenosine (m6A) Rabbit Polyclonal Antibody

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing; DB, Dot Blot.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB	IP	IF	IHC	ICC	FC	ChIP	ChIP-seq
-	1:50-1:200	-	-	-	-	-	1:500-1:2000

抗体详细信息如下::

About this Antibody
Name	N6-Methyladenosine (m6A) Rabbit Polyclonal Antibody
Category	Rabbit Polyclonal Antibody (pAb); Primary antibody
Isotype	IgG
Purification method	Affinity purification
Positive samples	Arabidopsis
Cellular location	-
Customer validation	-
About the Immunogen
Immunogen	Chemical/ Small Molecule corresponding to N6-methyladenosine (m6A) conjugated to keyhole limpet haemocyanin.
Sequence	-
Gene ID	-
Swiss Prot	-
Synonyms	m6A; N6-methyladenosine
Category	Epigenetics; RNA Editing/Splicing; Translational Control; ESC Pluripotency and Differentiation
Background	N6-methyladenosine (m6A) is a post-transcriptional modification found in various RNA subtypes. While the presence of m6A in RNA was described decades ago, the lack of tools has made interrogating the epitranscriptomic landscape challenging. With the emergence of new technologies such as miCLIP and NG-RNA-seq, researchers have been able to show that m6A is a biologically relevant mark in mRNA that is enriched in 3’ UTRs and stop codons. The m6A writer complex consists of a core heterodimer of methyltransferase-like protein 3 (METTL3) and methytransferase-like protein 14 (METTL14), and the additional regulatory proteins Virlizer/VIRMA and Wilms tumor 1-associated protein (WTAP). METTL3 is the catalytic methyltransferase subunit and METTL14 is the target recognition subunit that binds to RNA. The Virilzer/VIRMA protein directs m6A methylation to the 3’ UTRs and stop codons, and WTAP targets the complex to nuclear speckles, which are sites of RNA processing. Less is known about readers and erasers of m6A, and while the fat mass and obesity-associated protein FTO was the first discovered m6A demethylase, subsequent studies demonstrated that this enzyme may prefer the closely related m6Am mark in vivo. ALKBH5 was later shown to be a bona fide m6A demethylase enzyme, contributing to the idea that the m6A modification is dynamically regulated. Readers of the m6A mark include the YTH protein family, which can bind to m6A and influence mRNA stability and translation efficiency. The m6A mark and machinery have been shown to regulate a variety of cellular functions, including RNA splicing, translational control, pluripotency and cell fate determination, neuronal function, and disease. The m6A writer complex has been linked to various cancer types including AML and endometrial cancers. Additionally, m6A has been implicated in resistance to chemotherapy.

包装清单：

产品编号	产品名称	包装
XY-7407	N6-Methyladenosine (m6A) Rabbit Polyclonal Antibody	50μl
XY-050	Western一抗稀释液	50ml
—	说明书	1份

保存条件：
N6-Methyladenosine (m6A) Rabbit Polyclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
 

使用说明：
请根据抗体的实际用途选择相应的使用方法。
1. Western检测： 
a. 按照推荐的稀释比例用信裕生物提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。 
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。
2. 免疫染色：
可以使用信裕生物生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片：

Fig. 1. The m6A rabbit polyclonal antibody are tested in Nucleotide Array against N6-methyladenosine and unmodified adenosine Oligomer 2 - unmodified adenosine Oligomer 3 - unmodified adenosine	Fig. 2. The m6A rabbit polyclonal antibody are tested in Nucleotide Array against total RNA of Arabidopsis.