APC anti-mouse F4/80

库存 ： 现货

英文名 ：APC anti-mouse F4/80

CAS号 ：Biolegend

供应商 ： 上海善然生物科技有限公司

保存条件 ： 低温

规格 ：100ug

APC anti-mouse F4/80 Antibody
Catalog# / Size 123115 / 25 µg
123116 / 100 µg
Clone BM8
Other Names EMR1, Ly71
Isotype Rat IgG2a, κ
Description F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor
(EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely
used as a murine macrophage marker, which is expressed on the majority of tissue
macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red
pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph
node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80
has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not
been identified, but it has been reported that F4/80 is required for induction of CD8 T cellsmediated
peripheral tolerance.
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Product Details
Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Murine macrophages
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with APC under optimal
conditions.
Concentration 0.2 mg/ml
Storage & Handling The F4/80 antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric
analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10 cells in
100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
6
Excitation Laser Red Laser (633 nm)
Application Notes Additional reported applications (for the relevant formats) include: immunohistochemical staining of
acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections , and Western blotting. 1,2 6,7
Application References
(PubMed link indicates
BioLegend citation)
1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
Product Citations
1. Prantner D, et al. 2009. Infect Immun. 77:5334. PubMed
2. Sun L, et al. 2009. FASEB J. 3.602777778. PubMed
3. Oatley J, et al. 2009. Development. 136:1191. PubMed
4. Karpurapu M, et al. 2011. Blood. 118:5255. PubMed
5. Prantner D, et al. 2011. Infect Immun. 79:3922. PubMed
6. Kim M, et al. 2012. J Immunol. 188:4158. PubMed
7. Malik M, et al. 2012. Proc Natl Acad Sci U S A. 109:6130. PubMed
8. Klezovich-Bénard M, et al. 2012. PLoS One. 8:e1002481. PubMed
9. Stubelius A, et al. 2012. Immunobiology. 217:751. PubMed
Version: 2 Revision Date: 10/25/2013 
10. Neimert-Andersson T, et al. 2011. Vaccine. 29:8965. PubMed
11. Horsle B 2013. Development. 140:1517. PubMed
12. Patankar Y, et al. 2013. Infect Immun . 81:2043. PubMed
RRID AB_893493 (BioLegend Cat. No. 123115)
AB_893481 (BioLegend Cat. No. 123116)
Antigen Details
Structure EGF-TM7 family member, 160 kD glycoprotein
Distribution Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus
and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of
dendritic cells
Function Induction of immunological tolerance
Cell Type Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID 13733
Related Protocols
Cell Surface Flow Cytometry Staining Protocol
Brilliant Violet 605™ anti-mouse F4/80, Purified anti-mouse F4/80, LEAF™ Purified anti-mouse F4/80, Biotin anti-mouse F4/80, FITC
anti-mouse F4/80, PE anti-mouse F4/80, PE/Cy5 anti-mouse F4/80, PE/Cy7 anti-mouse F4/80, APC/Cyanine7 anti-mouse F4/80, Alexa
Fluor® 488 anti-mouse F4/80, Alexa Fluor® 647 anti-mouse F4/80, Pacific Blue™ anti-mouse F4/80, PerCP anti-mouse F4/80,
PerCP/Cyanine5.5 anti-mouse F4/80, Alexa Fluor® 700 anti-mouse F4/80, Brilliant Violet 421™ anti-mouse F4/80, Brilliant Violet 510™
anti-mouse F4/80, Alexa Fluor® 594 anti-mouse F4/80, Brilliant Violet 785™ anti-mouse F4/80, Purified anti-mouse F4/80 (Maxpar®
Ready), PE/Dazzle™ 594 anti-mouse F4/80, Brilliant Violet 650™ anti-mouse F4/80, Brilliant Violet 711™ anti-mouse F4/80,
APC/Fire™ 750 anti-mouse F4/80, TotalSeq™-A0114 anti-mouse F4/80, TotalSeq™-B0114 anti-mouse F4/80, TotalSeq™-C0114 antimouse
F4/80
Other Formats
Product Data
Thioglycolate-elicited BALB/c mouse
peritoneal macrophages stained with
BM8 APC
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violations that may occur with the use of our products.
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