ATG13 Rabbit Polyclonal Antibody

供应商 ：上海信裕生物科技有限公司

库存 ：50

英文名 ：ATG13 Rabbit Polyclonal Antibody

运输方式 ：常温

规格 ：50μl

ATG13 Rabbit Polyclonal Antibody

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB	IP	IF	IHC	ICC	FC	ChIP	ChIP-seq
1:500-1:2000	-	1:50-1:200	1:50-1:200	1:50-1:200	-	-	-

抗体详细信息如下::

About this Antibody
Name	ATG13 Rabbit Polyclonal Antibody
Category	Rabbit Polyclonal Antibody (pAb); Primary antibody
Isotype	IgG
Purification method	Affinity purification
Positive samples	HeLa, A-549, NCI-H460, SH-SY5Y, Rat brain, Rat heart
Cellular location	Cytoplasm, Preautophagosomal structure, cytosol
Customer validation	-
About the Immunogen
Immunogen	Recombinant fusion protein of human ATG13 (NP_055556.2).
Sequence	VHGTQADQERLATCTPSDRTHCAATPSSSEDTETVSNSSEGRASPHDVLETIFVRKVGAFVNKPINQVTLTSLDIPFAMFAPKNLELEDTDPMVNPPDSPETESPLQGSLHSDGSSGGSSGNTHDDFVMIDFKPAFSKDDILPMDLGTFYREFQNPPQLSSLSIDIGAQSMAEDLDSLPEKLAVHEKNVREFDAFVETLQ
Gene ID	9776
Swiss Prot	O75143
Synonyms	ATG13; KIAA0652; PARATARG8; Apg13; autophagy related 13
Category	Autophagy Signaling; mTOR Signaling; PI3K/Akt Signaling
Background	Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation, but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagyrelated (Atg) genes. Atg13/Apg13 was originally identified in yeast as a constitutively expressed protein that was genetically linked to Atg1/Apg1, a protein kinase required for autophagy. Over-expression of Atg1 suppresses the defects in autophagy observed in Atg13 mutants. Autophagy requires a direct association between Atg1 and Atg13, and is inhibited by TOR-dependent phosphorylation of Atg13 under high nutrient conditions. Similarly, mammalian Atg13 forms a complex with the Atg1 homologues ULK1/2, along with FIP200, which localizes to autophagic isolation membranes, and regulates autophagosome biogenesis. mTOR phosphorylates both Atg13 and ULK1, suppressing ULK1 kinase activity and autophagy. ULK1 can directly phosphorylate Atg13 at a yet unidentified site, presumably to promote autophagy. Additional studies suggest that Atg13 and FIP200 can function independently of ULK1 and ULK2 to induce autophagy through an unknown mechanism.

包装清单：

产品编号	产品名称	包装
XY-6249	ATG13 Rabbit Polyclonal Antibody	50μl
XY-050	Western一抗稀释液	50ml
—	说明书	1份

保存条件：
ATG13 Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
 

使用说明：
请根据抗体的实际用途选择相应的使用方法。
1. Western检测： 
a. 按照推荐的稀释比例用信裕生物提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。 
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。
2. 免疫染色：
可以使用信裕生物生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片：

Fig. 1. Western blot analysis of extracts of various cell lines, using ATG13 antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A) . Exposure time:1s.	Fig. 2. Immunohistochemistry of paraffin-embedded rat testis using ATG13 antibody at dilution of 1:100 (40x lens).
Fig. 3. Immunohistochemistry of paraffin-embedded rat brain using ATG13 antibody at dilution of 1:100 (40x lens).	Fig. 4. Immunofluorescence analysis of A549 cells using ATG13 antibody . Blue: DAPI for nuclear staining.