PeproTech重组人R-Spondin-1 20ug

价格： ￥2780

品牌：PeproTech 品牌认证

货号：120-38-20

产品详情

Recombinant Human R-Spondin-1

Product Details

Catalog Number:120-38
Description:

R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in the adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/β-catenin signaling pathway. Recombinant Human R-Spondin-1 is a 26.7 kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS-PAGE analysis under reducing conditions.

Source:CHO cells

Synonyms:Roof plate-specific spondin-1, RSPO1

AA Sequence:SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA

Purity:≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity:Assay #1: R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The expected ED₅₀ is 1.0-3.0 μg/ml.

Assay #2: Measured by its ability to induce Topflash reporter activity in HEK293 TCF/LEF Luc reporter cells. The ED₅₀ for this effect is typically 0.6-1.0 μg/ml in the presence of 50 ng/ml recombinant human BMP-2.

Note:

1mg will be provided as 2x500μg.

Cross Reactivity:
Human , Mouse