Anti-OGT/O-GlcNAc transferase抗体

供应商 ：上海西格

库存 ：43

靶点 ：详见说明书

级别 ：详见说明书

目录编号 ：详见说明书

克隆性 ：多克隆

抗原来源 ：Rabbit

保质期 ：详见说明书

抗体英文名 ：Anti-OGT/O-GlcNAc transferase

抗体名 ：O位N-乙酰葡萄糖胺OGT抗体

标记物 ：详见说明书

宿主 ：详见说明书

适应物种 ：详见说明书

免疫原 ：KLH conjugated synthetic peptide derived from human O-GlcNAc transferase

亚型 ：IgG

形态 ：详见说明书

应用范围 ：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

浓度 ：1mg/1ml 

保存条件 ：详见说明书

规格 ：0.1ml/100μg  0.2ml/200μg  

实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。

产品名称	O位N-乙酰葡萄糖胺OGT抗体
英文名称	Anti-OGT/O-GlcNAc transferase抗体
货号	XGK0478

英文名称  Anti-OGT/O-GlcNAc transferase
中文名称  O位N-乙酰葡萄糖胺OGT抗体
别    名  HRNT1; MGC22921; O-GlcNAc transferase; O GlcNAc; O-Linked N-Acetylglucosamine Transferase; O GlcNAc transferase p110 subunit; O GlcNAc transferase subunit p110; O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase); O linked N acetylglucosamine transferase 110 kDa subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT; OGT1_HUMAN; UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase.
浓    度  1mg/1ml
规 格   0.2ml/200μg    
抗体来源  Rabbit
克隆类型  polyclonal
交叉反应  Human, Mouse, Rat, Cow 
产品类型  一抗
研究领域  神经生物学 信号转导 转录调节因子 Alzheimer's
蛋白分子量  predicted molecular weight: 117kDa 
性    状  Lyophilized or Liquid 
免 疫 原   KLH conjugated synthetic peptide derived from human O-GlcNAc transferase
亚    型  IgG
纯化方法   affinity purified by Protein A
储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
产品介绍  Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related; the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.
Function : Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues.
Isoform 2, the mitochondrial isoform (mOGT), is cytotoxic and triggers apoptosis in several cell types including INS1, an insulinoma cell line.
Subunit : Heterotrimer; consists of one 78 kDa subunit and two 110 kDa subunits dimerized via TPR repeats 6 and 7. Interacts (via TPR repeats 6 and 7) with ATXN10 (By similarity). Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, HCFC1, PPP1CC and ACTB. Component of a THAP1/THAP3-HCFC1-OGT complex. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts directly with HCFC1; the interaction O-glycosylates HCFC1, regulates its proteolytic processing and transcriptional activity and, in turn, stabilizes OGT in the nucleus. Interacts (via TPRs 1-6) with SIN3A; the interaction mediates transcriptional repression in parallel with histone deacetylase.
Subcellular Location : Isoform 2: Mitochondrion. Membrane. Note=Associates with the mitochondrial inner membrane. Isoform 3: Cytoplasm. Nucleus. Cell membrane. Note=Mostly in the nucleus. Retained in the nucleus via interaction with HCFC1. After insulin induction, translocated from the nucleus to the cell membrane via phophatidylinisotide binding. Colocalizes with AKT1 at the plasma membrane. Isoform 4: Cytoplasm. Nucleus.
Tissue Specificity : Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
Post-translational modifications : Ubiquitinated, leading to its proteasomal degradation.
DISEASE : Note=Regulation of OGT activity and altered O-GlcNAcylations are implicated in diabetes and Alzheimer disease. O-GlcNAcylation of AKT1 affects insulin signaling and, possibly diabetes. Reduced O-GlcNAcylations and resulting increased phosphorylations of MAPT/TAU are observed in Alzheimer disease (AD) brain cerebrum.
Similarity : Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats.


抗体制备过程：
1.材料与试剂
  a．提取的动物 Ig
  b．弗氏完全佐剂和弗氏不完全佐剂
  c．青霉素和链霉素
  d．实验动物 兔
  e．其它材料及试剂
2、选择实验活体。
3、进行动物免疫实验。
4、试取血样进行测试，查看免疫效果。
5、如果免疫成功，杀死实验活体，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。胎牛血清（无菌采制）
我司提供以下技术外包服务:
1. Anti-OGT/O-GlcNAc transferase抗体分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等
2.蛋白工程：原核、哺乳动物蛋白表达系统等
3.病毒包装：腺病毒、慢病毒等
4.抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
5.细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。
Anti-Synapsin II /FITC 荧光素标记神经突触素2抗体IgGMulti-class antibodies规格： 0.2ml
HIV-1(gp41) 艾滋病病毒(抗原)Multi-class antibodies规格： 0.5mg
P选择素抗体 Anti-P-selectin 0.1ml
SATB1 英文名称： 核基质结合区结合蛋白1抗体 0.1ml
Escherichka coli K99 英文名称： 猪源产肠毒素性大肠杆菌K99抗体 0.2ml
Rhesus antibody Rh Phospho-Met(Tyr1349) 0酸化肝细胞生长因子受体(原癌基因)抗体 规格 0.1ml
HIV-1(gp41) 艾滋病病毒(抗原)Multi-class antibodies规格： 0.5mg
Anti-RRM1/FITC 荧光素标记抗核糖核苷酸还原酶1抗体IgGMulti-class antibodies规格： 0.2ml
IGF-II( Insulin-like Growth Factor-II ) 胰岛素样生长因子-II(抗原)Multi-class antibodies规格： 0.5mg
黑素瘤抗原-1抗体 Anti-MAGE-1/HLA-A1 protein 0.2ml
SLC27A2 英文名称： 长链脂肪酸辅酶A连接酶抗体 0.1ml
FSTL5 英文名称： 卵泡抑素相关蛋白5抗体 0.2ml
Rhesus antibody Rh Phospho-Pin1 (Ser16) 0酸化肽基脯氨酞顺反异构酶Pinl抗体 规格 0.1ml
IGF-II( Insulin-like Growth Factor-II ) 胰岛素样生长因子-II(抗原)Multi-class antibodies规格： 0.5mg
PCNA 人增殖细胞核抗原Multi-class antibodies规格： 48T
Anti-CX3CL1 CX3CL1抗体Multi-class antibodies规格： 0.1ml
Rhesus antibody Rh NGN1/Neurogenin 1 神经元素1抗体 规格 0.2ml
Human recombination activating gene 1,RAG-1 ELISA Kit 人重组激活基因1 96T
TRPC3 英文名称： 色酸相关蛋白3抗体 0.1ml
CHM 英文名称： 遗传性脉络膜缺乏症相关蛋白抗体 0.1ml
Anti-CX3CL1 CX3CL1抗体Multi-class antibodies规格： 0.1ml
人α1微球蛋白(α1-MG)ELISA 试剂盒 96T/48T 试剂盒 组装/原装
兔子β血小板球蛋白/β血栓环蛋白(β-TG)免疫试剂盒 Rabbit β-Thromboglobulin,β-TG ELISA Kit
热休克蛋白60(Hsp-60)ELISA试剂盒 ，英文名： Hsp-60 ELISA Kit
Rabbit50%complemehemolysis,CH50ELISA试剂盒兔血清总补体(CH50)ELISA试剂盒规格：96T/48T
Humanai-chorionicgonadoopin-aibody,AhCGAbELISA试剂盒人抗人绒毛膜促性腺激素抗体(AhCGAb)ELISA试剂盒规格：96T/48T
HumanB-LymphocyteChemoatacta1,BLC-1ELISAKit人B-淋巴细胞趋化因子1(BLC-1/CXCL13)ELISA试剂盒规格：96T/48T
Anti-OGT/O-GlcNAc transferase抗体人富含半胱酸型酸性蛋白(SPARC)ELISA试剂盒 96T/48T 试剂盒 组装/原装
小鼠β淀粉样蛋白1-42(Aβ1-42)免疫试剂盒 Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
细胞周期蛋白A1(CCNA1)ELISA试剂盒 ，英文名： CCNA1 ELISA Kit
Mouseleukemiainhibitoryfactorreceptor,LIFRELISA试剂盒小鼠白血病抑制因子受体(LIFR)ELISA试剂盒规格：96T/48T
ELISAKitIL-1sRⅠ兔子白介素1可溶性受体I规格：48T/96T
DeerEndothelialniicoxidesyhase,eNOSELISAKit鹿内皮型合成酶(eNOS)ELISA试剂盒规格：96T/48T
抗体的鉴定：
1）抗体的效价鉴定：鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
3）真核翻译起始因子2C2抗体的亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。