Phospho-Cdc6 (Ser54) Rabbit Monoclonal Antibody

规格 ：50μl

产品编号	产品名称	产品包装	产品价格
AF2755	Phospho-Cdc6 (Ser54) Rabbit Monoclonal Antibody	50μl	709.00元

来源	用途	交叉反应性	分子量
Rabbit	WB, IF, ICC	H	63.0kDa

       WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
       H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
       配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
       建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB	IP	IF	IHC	ICC	FC	ELISA	ChIP
1:1,000-1:2,000	-	1:50-1:200	-	1:50-1:200	-	-	-

抗体详细信息如下:

About this Antibody
Name	Phospho-Cdc6 (Ser54) Rabbit Monoclonal Antibody
Category	Rabbit Monoclonal Antibody (RabMAb); Primary antibody
Isotype	IgG
Purification	Affinity purification
About the Immunogen
Immunogen	Recombinant protein
Gene ID	990(Human)
SwissProt	Q99741(Human)
Synonyms	CDC18L; HsCDC18; HsCDC6
Category	Cell Cycle
Background	Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein Tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory Tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (x complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

包装清单:

产品编号	产品名称	包装
AF2755	Phospho-Cdc6 (Ser54) Rabbit Monoclonal Antibody	50μl
AZ050	Western一抗稀释液	50ml
-	说明书	1份

保存条件:
       Phospho-Cdc6 (Ser54) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
       如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
       回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
       提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
       本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
       为了您的安全和健康，请穿实验服并戴一次性手套操作。