Total Exosome RNA & Protein Isolation Kit

库存 ：10

英文名 ：Total Exosome RNA & Protein Isolation Kit

供应商 ：上海觅拓生物

保存条件 ：Store at 2 to 8 °C

描述

The Total Exosome RNA and Protein Isolation Kit enables isolation of total RNA and proteins from exosome samples extracted from biological fluids (e.g., by using Total Exosome Isolation (from cell culture media) or Total Exosome Isolation (from serum)).
• Extracts highly pure total RNA (including small RNA fraction) from exosomes
• Recovers protein and RNA from the same sample
• Compatible with all protocols for exosome isolation

The Total Exosome RNA and Protein Isolation Kit is designed for the isolation of both RNA and protein from pre-isolated or enriched exosomes (not supplied). It is suitable for studies of RNA expression (specifically miRNA), processing, or function. The kit allows recovery of both RNA and proteins from the same purified exosome preparation. One portion of the sample is subjected to organic extraction, followed by immobilization of RNA on glass-fiber filters to purify total RNA or enrich for small RNA species. The other portion is then used directly for common protein applications such as Western blotting.

High yields of ultra-pure RNA can be prepared in about 30–60 minutes. The RNA can then be used in a variety of downstream applications, including qRT-PCR, high-throughput sequencing (e.g., using the Ion Torent™ PGM™, Ion Torrent™ Proton™, or SOLiD® sequencing instruments), microarray analysis, solution hybridization assays, and blot hybridization.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

规格

Sample Type (General):	Organelle & Vesicle-Related Samples
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Downstream Application:	Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Western Blotting, microRNA Analysis
Final Product:	Protein, Total RNA, micro RNA
Isolation Technology:	Organic Extraction, Spin Column (Glass Fiber Filter)
Product Size:	40 preps
Sample Type (Specific):	Exosomes
Shipping Condition:	Room Temperature

内容及储存

Store at 2 to 8 °C.