Jackson/Alkaline Phosphatase AffiniPure Rabbit Anti-Chicken IgY (IgG) (H+L)/1.0 ml/303-055-003

库存 ：100

保质期 ：一年

克隆性 ：多克隆

• 商品编号：303-055-003
• 品牌：Jackson
• 公司：Jackson ImmunoResearch Laboratories
• 公司分类：Whole IgG Affinity-Purified Antibodies
• Target：Chicken
• Host：Rabbit
• Specificity：IgY (IgG) (H+L)
• Conjugate：Alkaline Phosphatase
• Unit：1.0 ml
• Antibody_Format：Whole IgG
• Clonality：Polyclonal
• 产地：美国

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithwholemoleculechickenIgY.Italsoreactswiththelightchainsofotherchickenimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.Theantibodymaycross-reactwithimmunoglobulinsfromotherspecies.
PhysicalState:Freeze-driedsolid
Storage:Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MTris-HCl,0.25MNaCl,pH8.0
Preservative:0.05%SodiumAzide

SuggestedWorkingConcentrationorDilutionRange:
ELISA:-1:5,000-1:50,000
WesternBlot:-1:5,000-1:50,000