人夹心法1000个因子半定量抗体芯片

价格：询价

品牌：raybiotech 品牌认证

货号：GSH-CAA-X00

产品详情

RayBio^® 抗体芯片:

将大量不同的抗体有序地排列、固定于固相载体表面，形成微阵列。利用抗原抗体间特异性结合的原理，实现对生物蛋白质分子精准、快速、高通量的检测。
（参加活动是是人同时检测1000个蛋白的产品）
Antigen Information

Gene Symbols:

Assay Format

Number of Targets Detected:

1000

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Semi-Quantitative:

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

NEED HELP RUNNING THIS ASSAY?
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Introduction

RayBio^® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody^®Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

High sensitivity (pg/mL to ng/mL levels)
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Test 16 samples on each slide
Data analysis software provides one-step computation

Target Names

17-beta-HSD1 (HSD17B1), 2B4 (CD244), 4-1BB (TNFRSF9/CD137), 4-1BB Ligand (TNFSF9), 5'-Nucleotidase (CD73), 6Ckine (CCL21), ACE, ACE-2, Activin A, Activin R1A (ALK-2), Activin R1B (ALK-4), Activin R2A, Activin R2B (ACVR2B), ADA, ADAM-12, ADAM-22, ADAM23, ADAM-8, ADAM-9, ADAMTS-13, ADAMTSL-1, Adiponectin (ACRP30), Adipsin (Complement Factor D), ADP-Ribosyl Cyclase 2 (BST-1/CD157), aFGF (FGF-1), Aggrecan, AgRP, AIF, AKR1C4, Albumin, ALCAM (CD166), ALK-1, ALK-6 (BMPR-IB), Alpha-fetoprotein, AMICA, AmiGO, AmiGO2, Aminopeptidase LRAP (ERAP2), Aminopeptidase P2 (XPNPEP2), Amnionless, Amphiregulin, AMSH (STAMBP), Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, Angiotensinogen (SERPINA8), ANGPTL3, ANGPTL4, ANGPTL7, Annexin A5 (Annexin V), APRIL, Arginase 1, ARNT (HIF-1 beta), Artemin, Arylsulfatase A (ARSA), Arylsulfatase B (ARSB), ASAH2 (Neutral Ceramidase), ASAHL, AXL, B3GNT2, B4GalT1, B7-H1 (CD274), B7-H2 (ICOS Ligand), B7-H3 (CD276), B7-H4, BAFF, BAFF R, BAI1, BAMBI (NMA), BATF3, BCAM, BCL-10, BCL-2, bcl-w, BCMA, BDNF, beta IG-H3, Beta-2 Microglobulin, Betacellulin (BTC), Beta-glucuronidase (GUSB), beta-NGF, bFGF, BID, Biglycan, BLAME (SLAMF8), BLC (CXCL13), BMP-2, BMP-4, BMP-5, BMP-7, BMP-8, BMP-9, BMPR-IA (ALK-3), BMPR-II, BOC (Brother of CDO), Bora, Brevican, Brorin (VWC2)...View all 1000 human target proteins

Application Notes

Suggested Application

Multiplexed Protein Detection
Detection of Relative Protein Expression
Detecting Patterns of Cytokine Expression
Biomarker Screening
Identifying Key Factors
Confirming a Biological Process

Kit Components

Antibody Array Slide/s
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
30 ml Centrifuge Tube
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with samples
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.