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库存 :231
英文名 :BstC8I
保质期 :长期
供应商 :北京百奥莱博科技有限公司
保存条件 :-20℃
规格 :100U
特别提示:包括BstC8I内切酶在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:BstC8I内切酶
英文名称:BstC8I
产品货号:GS2207
产品规格:100U
储存条件:冷冻(-20℃)
使用说明
At 37℃ activity is 50%from maximum.
技术规格
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 55℃ in a total reaction volume of 50μl. |
Recognition sites | GCN↑NGC |
来源 | Bacillus stearothermophilus C8 |
Assayed on | Lambda DNA |
Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
Optimal temperature | 55℃ |
Storage conditions | 10 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;10 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃. |
Ligations | After 5-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 55℃. |
Methlation effect | Methylation sensitivity not tested |
Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
Campatible Ends | Cac8I |
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