PspOMI内切酶北京品牌

库存 ：399

英文名 ：PspOMI

保质期 ：长期

供应商 ：北京百奥莱博科技有限公司

保存条件 ：-20℃

规格 ：1.5kU

特别提示：包括PspOMI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：PspOMI内切酶
英文名称：PspOMI
产品货号：GS2161
产品规格：1.5kU

储存条件：冷冻（-20℃）

使用说明

The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.13.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(BamHI-digest)in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	G↑GGCCC
来源	E.coli strain that carries the cloned PspOM I gene from Pseudomonas species OM2164
Assayed on	Lambda DNA(BamHI-digest)
Optimal buffer	Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	20 mM Tris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;and 50%glycerol.Store at -20℃.
Ligations	After 10-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	ApaI^,Bsp120I

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