造血细胞信号转导蛋白抗体价格_品牌:gelatins-丁香通官网

供应商：江西江蓝纯生物试剂有限公司

库存：153

克隆性：单克隆

保质期：1年

抗体英文名：HCST

抗体名：造血细胞信号转导蛋白抗体

适应物种：人/动物/植物

应用范围：WB,ELISA等

浓度：1mg/ml

保存条件：-20 °

规格：100ul/200ul

产品货号 : jlcR23084
英文名称 : HCST
中文名称 : 造血细胞信号转导蛋白抗体
别    名 : DAP10; DNAX activation protein 10; Hematopoietic cell signal transducer; KAP10; Membrane protein DAP10; phosphoinositide 3 kinase adaptor protein; PIK3AP; Transmembrane adapter protein KAP10; HCST_HUMAN.
研究领域 : 免疫学信号转导
抗体来源 : Rabbit
克隆类型 : Polyclonal
交叉反应 :   Human,
产品应用 : WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分子量 : 8kDa
细胞定位 : 细胞膜
性    状 : Lyophilized or Liquid
浓    度 : 1mg/ml
免疫原 : KLH conjugated synthetic peptide derived from human HCST:11-50/93 <Extracellular>
亚    型 : IgG
纯化方法 : affinity purified by Protein A
储存液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 :   This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Function:
Transmembrane adapter protein which associates with NKG2D to form an activation receptor NKG2D-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with NKG2D-DAP10 triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full NKG2D-HCST-mediated activation and ultimate killing of target cells. In NK cells, NKG2D-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. NKG2D-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express NKG2D ligands escape from immune surveillance mediated by NK cells.

Subunit:
Interacts with CLEC5A. Forms an CLEC5A/TYROBP/HCST trimolecular complex depending almost solely on TYROBP. Homodimer; Disulfide-linked. Interacts with NKG2D to form a stable complex, which results in surface expression of both proteins, whereas alone, it is minimally expressed. Interacts with PIK3R1 and GRB2.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Predominantly expressed in hemopoietic cells such as NK cells, subset of T-cells and monocytes. Detected in leukocytes, spleen, and thymus.

Post-translational modifications:
Phosphorylated; PIK3R1 and GRB2 associate specifically with tyrosine-phosphorylated HCST.
O-glycosylated.

Similarity:
Belongs to the DAP10 family.

SWISS:
Q9Y3Y0

Gene ID:
10870

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片