Phospho-p63 (Ser160/162) Antibody价格_品牌:Cell Signaling Technology-丁香通官网

抗体英文名：Phospho-p63 (Ser160/162) Antibody

抗原：synthetic phosphopeptide corresponding to residues surrounding Ser160/160 of human TAp63-alpha

应用范围：W, IHC-P, F

宿主：Rabbit

适应物种：H,M,R,C,X

库存：大量

供应商：CST

保质期：详见说明书

级别：详见MSDS文件

是否单克隆：2

保存条件：-20°c

规格：100 ul (10 western blots)/carrier free & custom formulation / quantity

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat C=Chicken X=Xenopus
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IHC-P F	H (M) (R) (C) (X)	Endogenous	75	Rabbit

Protocols	4981: Flow, IHC / Paraffin, Western Blotting
Specificity / Sensitivity	Phospho-p63 (Ser160/162) Antibody detects endogenous levels of p63 only when phosphorylated at serine 160/162.
Source / Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser160/160 of human TAp63-alpha. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting Western blot analysis of extracts from untreated or nocodazole-treated (50 ng/ml, 24 h) ME-180 cells using Phospho-p63 (Ser160/162) Antibody (upper) or p63alpha Antibody #4892 (lower). IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the esophagus, showing nuclear localization, using Phospho-p63 (Ser160/162) Antibody. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded ME-180 cells, untreated (left) or nocodozole-treated (right), showing induced nuclear staining, using Phospho-p63 (Ser160/162) Antibody. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma, using Phospho-p63 (Ser160/162) Antibody in the presence of control peptide (left) or antigen-specific peptide (right). Flow Cytometry Flow cytometric analysis of Jurkat cells using Phospho-p63 (Ser160/162) antibody versus propidium iodide (DNA content).
Background	The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription intiation site that yields a 40 kDa deltaNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the alpha, beta and gamma isoforms (3,4). TAp63-alpha (full-length) contains multiple serine residues followed by proline (Ser-Pro motif) that are potential cdk substrates expected to be phosphorylated in mitosis. Among these are Ser160, Ser162, Ser395, and Ser455. Levine, A. J. et al. (1997) Cell 88, 323-331. Waltermann, A. et al. (2003) Oncogene 22, 5686-5693. Hibi, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5462-5467. Yang, A. et al. (1999) Nature 398, 714-718.
Application References	Westfall, M. D. et al. (2005) Ultraviolet Radiation Induces Phosphorylation and Ubiquitin-Mediated Degradation of deltaNp63alpha. Cell Cycle 4 (5), 710-716. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products	4892 p63-α Antibody 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody 7074 Anti-rabbit IgG, HRP-linked Antibody 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7727 Biotinylated Protein Ladder Detection Pack 7003 20X LumiGLO^® Reagent and 20X Peroxide For Research Use Only. Not For Use In Diagnostic Procedures.