GSK-3α (D80E6) Rabbit mAb价格_品牌:Cell Signaling Technology-丁香通官网

抗体英文名：GSK-3α (D80E6) Rabbit mAb

抗原：synthetic peptide corresponding to the sequence of human GSK-3α protein

应用范围：W, IP

适应物种：H,M,R,Hm,Mk

供应商：CST

保质期：详见说明书

库存：大量

级别：详见MSDS文件

是否单克隆：1

保存条件：-20°c

规格：40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP	H M R Hm Mk	Endogenous	51	Rabbit IgG

Protocols	4337: Immunoprecipitation, Western Blotting
Specificity / Sensitivity	GSK-3α (D80E6) Rabbit mAb detects endogenous levels of total GSK-3α protein. The antibody does not cross-react with GSK-3β.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GSK-3α protein. Western Blotting Western blot analysis of extracts from various cell types using GSK-3α (D80E6) Rabbit mAb.
Background	Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). GSK-3α regulates the production of amyloid-β peptides, a major component of the plaques that accumulate with progression of Alzheimer disease. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates amyloid-β production by specifically inhibiting the cleavage of amyloid precursor protein (APP) by γ-secretase, blocking accumulation of amyloid-β peptides in the brains of mice that overproduce APP (6). Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279. Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141. Cross, D.A. et al. (1995) Nature 378, 785-789. Nusse, R. (1997) Cell 89, 321-323. Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511. Phiel, C.J. et al. (2003) Nature 423, 435-439.
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