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抗体英文名 :Phospho-APP (Thr668) (D90B8) Rabbit mAb
抗原 :synthetic peptide corresponding to residues surrounding Thr668 of human APP protein
应用范围 :W, IP
保质期 :详见说明书
库存 :大量
适应物种 :H,M,R
级别 :详见MSDS文件
供应商 :CST
是否单克隆 :1
保存条件 :-20°c
规格 :100 ul (10 western blots)/carrier free & custom formulation / quantity
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
---|---|---|---|---|
W IP | H M R | Endogenous | 100-140 | Rabbit IgG |
Protocols | |
---|---|
Specificity / Sensitivity | Phospho-APP (Thr668) (D90B8) Rabbit mAb detects different isoforms of endogenous amyloid β (A4) precursor protein only when phosphorylated at Thr668 (or the corresponding position of other isoforms). |
Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr668 of human APP protein. |
Background | Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (Aβ), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (AD) (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle-dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).
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Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know! |
Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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入驻年限:11年