PINK1 (D8G3) Rabbit mAb

价格：询价

品牌：Cell Signaling Technology 品牌认证

货号：6946

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抗体英文名：PINK1 (D8G3) Rabbit mAb

抗原：synthetic peptide corresponding to residues surrounding Pro140 of human PINK1 protein

应用范围：W, IP

适应物种：H

供应商：CST

保质期：详见说明书

级别：详见MSDS文件

库存：大量

是否单克隆：多克隆

保存条件：-20°c

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP	H	Endogenous	60, 50	Rabbit IgG

Protocols	6946: Immunoprecipitation , Western Blotting
Specificity / Sensitivity	PINK1 (D8G3) Rabbit mAb recognizes endogenous levels of total PINK1 protein.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro140 of human PINK1 protein. Western Blotting Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a cDNA construct expressing full-length human PINK1 (hPINK1, +) using PINK1 (D8G3) Rabbit mAb. Western Blotting Western blot analysis of extracts from HeLa cells, untreated (-) or treated with CCCP (10 μM, 24 hr; +), using PINK1 (D8G3) Rabbit mAb.
Background	PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria (1-3). PINK1 phosphorylates Parkin and promotes its translocation to mitochondria (2). Research studies have shown that mutations in PINK1 are linked to autosomal recessive early onset Parkinson’s disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction (1,3). Liu, W. et al. (2009) PLoS One 4, e4597. Kim, Y. et al. (2008) Biochem Biophys Res Commun 377, 975-80. Petit, A. et al. (2005) J Biol Chem 280, 34025-32.
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