CCT2 Antibody

价格：询价

品牌：Cell Signaling Technology 品牌认证

货号：3561

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抗体英文名：CCT2 Antibody

抗原：synthetic peptide corresponding to human CCT2

应用范围：W, IP

宿主：Rabbit

适应物种：H,M,R,Mk

保质期：详见说明书

供应商：CST

库存：大量

级别：详见MSDS文件

是否单克隆：2

保存条件：-20°c

规格：100 ul (10 western blots)/carrier free & custom formulation / quantity

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R Mk	Endogenous	54	Rabbit

Protocols	3561: Immunoprecipitation, Western Blotting
Specificity / Sensitivity	CCT2 Antibody detects endogenous levels of total CCT2 protein.
Source / Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human CCT2. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting Western blot analysis of extracts from various cell lines using CCT2 Antibody.
Background	CCT2 is one of eight largely unrelated subunit proteins found in a protein chaperone complex known as the chaperonin-containing TCP-1 (CCT) or TRiC complex. The CCT complex is an abundanct cytoslic component that is credited with helping newly synthesized polypeptides adopt the correct conformation (1). Proteins that fold and assemble with the help of CCT include the cytoskeletal proteins actin and tubulin as well as up to 15% of newly synthesized eukaryotic proteins (2). CCT2 is the β-subunit of the chaperone complex and is one of several CCT proteins that exhibit increased expression in response to stress. This implies that the CCT complex helps cells recover from protein damage by assisting in protein folding and assembly (3). CCT subunit levels also change throughout the cell cycle, with lower proteins levels (and reduced chaperone activity) found during induced cell cycle arrest during at M phase (4). Each CCT subunit is thought to perform a specific function during protein folding and assembly (5); CCT2 exhibits both actin and tubulin binding activities (6,3) but the exact molecular function on this subunit remains uncertain. Kubota, H. et al. (1995) Eur J Biochem 230, 3-16. Valpuesta, J.M. et al. (2002) FEBS Lett 529, 11-6. Yokota, S.I. et al. (2000) Eur J Biochem 267, 1658-64. Yokota, S. et al. (2001) Eur J Biochem 268, 4664-73. Kubota, H. et al. (1994) Curr Biol 4, 89-99. McCormack, E.A. et al. (2001) J Struct Biol 135, 198-204.
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