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抗体英文名 :Pim-3 (D17C9) Rabbit mAb
抗原 :synthetic peptide corresponding to residues surrounding Ser275 of human Pim-3
应用范围 :W, IP
适应物种 :H,M,R,Mk
供应商 :CST
库存 :大量
保质期 :详见说明书
级别 :详见MSDS文件
是否单克隆 :1
保存条件 :-20°c
规格 :40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
---|---|---|---|---|
W IP | H M R (Mk) | Endogenous | 35 | Rabbit IgG |
Protocols | |
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Specificity / Sensitivity | Pim-3 (D17C9) Rabbit mAb detects endogenous levels of total Pim-3 protein. It does not cross-react with other Pim family members. |
Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser275 of human Pim-3. Western BlottingWestern blot analysis of extracts from COS-7 cells, untransfected () or transfected with a mouse Pim-3 construct (+), using Pim-3 (D17C9) Rabbit mAb. Western BlottingWestern blot analysis of recombinant Pim-1, 2, and 3 using Pim-3 (D17C9) Rabbit mAb. Western BlottingWestern blot analysis of extracts from various cell lines using Pim-3 (D17C9) Rabbit mAb. |
Background | Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12). Pim-3, originally named Kid-1, was identified as a protein induced by depolarization in PC-12 cells (13). Like other family members, Pim-3 can phosphorylate Bad to inhibit Bad-mediated apoptosis (14). Aberrant expression of Pim-3 has been observed in a number of types of cancer, including liver, pancreas, colon and gastric cancer (14-17).
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Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know! |
Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
美国CST中国公司(丁香通商铺)
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入驻年限:11年