Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb价格_品牌:Cell Signaling Technology-丁香通官网

抗体英文名：Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb

抗原：synthetic peptide corresponding to the amino terminus of histone H3 in which Lys4 is tri-methylated

应用范围：W, IHC-P, IF-IC, ChIP

保质期：详见说明书

供应商：CST

适应物种：H,M,R,Mk,Dm,Sc,X,Z

级别：详见MSDS文件

库存：大量

是否单克隆：单克隆

保存条件：-20°c

规格：100 ul (10 western blots)/carrier free & custom formulation / quantity

20190715-0926 期间，购买任一ChIP试剂盒，可0元得该 T(20ul) 包装抗体或礼品！

促销活动详情：

促销时间
2019年7月15日—2019年9月26日

优惠一
购买任一ChIP试剂盒（见下表1），将特别赠送经ChIP或ChIP-seq验证的小包装抗体一支（见下表2）或精美礼品一份（见优惠二）。

促销试剂盒列表1

货号	产品名称	应用
56383S	SimpleChIP® Plus Sonication Chromatin IP Kit（超声法，磁珠）	ChIP ChIP-seq
9005S	SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)（酶解法，磁珠）	ChIP ChIP-seq
9004S	SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)（酶解法，琼脂糖微珠）	ChIP
9003S	SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)（酶解法，磁珠）	ChIP ChIP-seq
9002S	SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)（酶解法，琼脂糖微珠）	ChIP
56795S	SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina®（DNA 文库构建）	ChIP-seq

赠送抗体列表2

优惠二

一次性购买促销抗体（优惠一中6个ChIP试剂盒除外）目录价格达7000元，即可获赠精美礼品一份。

查询参加促销的具体产品，点击此处或微信公众号「CST博士互助平台」后台回复“促销” ，了解促销详情。

#9751T Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb 产品详情：

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster X=Xenopus Z=Zebrafish Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IHC-P IF-IC ChIP	H M R Mk Dm Sc (X) (Z)	Endogenous	17	Rabbit IgG

Protocols	9751: ChIP Agarose , ChIP Magnetic , IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity	Tri-Methyl-Histone H3 (Lys4) Antibody detects endogenous levels of histone H3 when tri-methylated on Lys4. This antibody shows some cross-reactivity with histone H3 that is di-methylated on Lys4, but does not cross-react with non-methylated or mono-methylated histone H3 Lys4. In addition, the antibody does not cross-react with methylated histone H3 Lys9, Lys27, Lys36 or methylated histone H4 Lys20.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys4 is tri-methylated. Western Blotting Antibody specificity was determined by Western blotting. HeLa and NIH/3T3 cell lysates were probed with Tri-Methyl Histone H3 (Lys4) (C42D8) Rabbit mAb (Panel A) or Tri-Methyl Histone H3 (Lys4) Rabbit mAb pre-adsorbed with 1.5 μM of various competitor peptides (Panels B-M). As shown, only the tri-methyl histone H3 (Lys4) peptide competed away binding of the antibody. Western Blotting Western blot analysis of various cell types using Tri-Methyl Histone H3 (Lys4) (C42D8) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Tri-Methyl Histone H3 (Lys4) (C42D8) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Tri-Methyl Histone H3 (Lys4) (C42D8) Rabbit mAb. IF-IC Confocal immunofluorescent analysis of HeLa cells using Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Chromatin IP Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ HeLa cells and either 10 μl of Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human RPL30 Exon 3 Primers #7014, SimpleChIP^® Human GAPDH Exon 1 Primers #5516, SimpleChIP^® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Background	The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9). Peterson, C.L. and Laniel, M.A. (2004) Curr. Biol. 14, R546-R551. Kubicek, S. et al. (2006) Ernst Schering Res. Found Workshop , 1-27. Lin, W. and Dent, S.Y. (2006) Curr. Opin. Genet. Dev. 16, 137-142. Lee, D.Y. et al. (2005) Endocr. Rev. 26, 147-170. Daniel, J.A. et al. (2005) Cell Cycle 4, 919-926. Shi, X. et al. (2006) Nature 442, 96-99. Wysocka, J. et al. (2006) Nature 442, 86-90. Wysocka, J. et al. (2005) Cell 121, 859-872. Trojer, P. and Reinberg, D. (2006) Cell 125, 213-217.
Application References	Pasini, D. et al. (2010) Nucleic Acids Res 38, 4958-69. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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