Rab6 (D37C7) Rabbit mAb价格_品牌:Cell Signaling Technology-丁香通官网

抗体英文名：Rab6 (D37C7) Rabbit mAb

抗原：recombinant protein specific to the carboxy terminus of human Rab6 protein

应用范围：W, IP, IF-IC

保质期：详见说明书

级别：详见MSDS文件

库存：大量

供应商：CST

适应物种：H,M,R,Mk

是否单克隆：1

保存条件：-20°c

规格：40 ul/100 ul (10 western blots)/carrier free & custom formulation / quantity

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IF-IC	H M R (Mk)	Endogenous	24	Rabbit

Protocols	9625: Immunofluorescence, Western Blotting
Specificity / Sensitivity	Rab6 (D37C7) Rabbit mAb recognizes endogenous levels of total Rab6 protein.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Rab6 protein. Western Blotting Western blot analysis of extracts from various cell lines using Rab6 (D37C7) Rabbit mAb. IF-IC Confocal immunofluorescent analysis of HeLa cells using Rab6 (D37C7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).
Background	Rab6 is a member of the Ras superfamily of small Rab GTPases implicated in endocytosis (1). The three distinct members of the Rab6 subfamily (Rab6A, Rab6A', and Rab6B) are structurally similar but likely exhibit non-overlapping functions (2,3). Rab6 localized to the Golgi (4) regulates retrograde transport of membrane-bound target proteins from the Golgi apparatus to the endoplasmic reticulum (5-7) or from the Golgi to the endosome during exocytotic transport (8). Rab6 interacts with microtubule motor proteins such as rabkinesin-6 (KIF20A) and dynein/dynactin complexes; Rab6-mediated transport requires a functionally intact microtubule system (9,10). Rab6 also regulates cytokinesis and cell cycle check point through interactions with Rab6 effector proteins, including the dynein/dynactin protein DCTN1 and the GTPase activating protein RABGAP1 (11,12). Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17. Echard, A. et al. (2000) Mol Biol Cell 11, 3819-33. Del Nery, E. et al. (2006) Traffic 7, 394-407. Goud, B. et al. (1994) Ann N Y Acad Sci 733, 340-3. White, J. et al. (1999) J Cell Biol 147, 743-60. Opdam, F.J. et al. (2000) J Cell Sci 113 ( Pt 15), 2725-35. Martinez, O. et al. (1997) Proc Natl Acad Sci USA 94, 1828-33. Grigoriev, I. et al. (2007) Dev Cell 13, 305-14. Echard, A. et al. (1998) Science 279, 580-5. Young, J. et al. (2005) Mol Biol Cell 16, 162-77. Hill, E. et al. (2000) EMBO J 19, 5711-9. Miserey-Lenkei, S. et al. (2006) EMBO J 25, 278-89.
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