Phospho-(Ser) PKC Substrate Antibody价格_品牌:Cell Signaling Technology-丁香通官网

抗体英文名：Phospho-(Ser) PKC Substrate Antibody

抗原：synthetic phospho-PKC substrate peptides

应用范围：W, IP, E-P

宿主：Rabbit

适应物种：All

级别：详见MSDS文件

库存：大量

保质期：详见说明书

供应商：CST

是否单克隆：2

保存条件：-20°c

规格：200 ul (10 western blots)/600 ul (30 western blots)/carrier free & custom formulation / quantity

more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Applications	Reactivity	Sensitivity	Source
W IP E-P	All	Endogenous	Rabbit

Protocols	2261: ELISA Peptide, Immunoprecipitation, Western Blotting
Specificity / Sensitivity	Phospho-(Ser) PKC Substrate Antibody detects endogenous levels of many cellular proteins only when phosphorylated at serine residues surrounded by Arg or Lys at the -2 and +2 positions and a hydrophobic residue at the +1 position. The antibody does not cross-react with nonphosphorylated serine residues, with phospho-threonine in the same motif, or with phospho-serine in other motifs. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification	Polyclonal antibodies are produced by immunizing animals with synthetic phospho-PKC substrate peptides . Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting Western blot analysis of extracts from Jurkat cells, untreated or treated with PMA (50 ng/ml) and ionomycin (1 µM for 20 minutes prior to lysis) and subjected to 2-D electrophoresis, using Phospho-(Ser) PKC Substrate Antibody. Western Blotting Western blot analysis of extracts from WEHI 231 cells and Jurkat cells, untreated or TPA-treated, using Phospho-(Ser) PKC Substrate Antibody. ELISA-Peptide Phospho-(Ser) PKC Substrate Antibody ELISA assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* denotes phosphorylated serine.)
Background	Although protein kinase C (PKC) family members are involved in a number of signal transduction processes including secretion, gene expression, proliferation, and muscle contraction (1,2), many of their substrates remain unidentified. Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC), and atypical PKCs (aPKC). PKCα, βI, βII, and γ isoforms belong to the cPKC group (1). When activated, cPKC isozymes phosphorylate substrates containing Ser or Thr, with Arg or Lys at the -3, -2, and +2 positions, and a hydrophobic amino acid at position +1 (1-3). To identify potential PKC substrates, Cell Signaling Technology has developed antibodies that recognize this motif. Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb is specific to cPKC substrates containing phospho-Ser. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. Obata, T. et al. (2000) J Biol Chem 275, 36108-15.
Application References	White, D. et al. (2007) J Exp Biol 210, 4053-64. Applications: Western Blotting Kim, J.Y. and Saffen, D. (2005) J Biol Chem 280, 32035-47. Applications: IP Western Blotting Smith, J.A. et al. (2005) Cancer Res 65, 1027-34. Applications: Western Blotting Iwabu, A. et al. (2004) J Biol Chem 279, 14551-60. Applications: Western Blotting Pierchala, B.A. et al. (2004) J Biol Chem 279, 27986-93. Applications: Western Blotting Jones, M.L. et al. (2004) J Biol Chem 279, 40475-83. Applications: Western Blotting Carpenter, L. et al. (2004) Diabetes 53, 53-60. Applications: Western Blotting Kohn, E.A. et al. (2003) Cancer Res 63, 31-5. Applications: Western Blotting Li, Y. et al. (2003) Mol Cell Biol 23, 6255-66. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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