FoxO1 (L27) Antibody价格_品牌:Cell Signaling Technology-丁香通官网

抗体英文名：FoxO1 (L27) Antibody

抗原：synthetic peptide corresponding to amino acids within the amino terminus of mouse FoxO1

应用范围：W

宿主：Rabbit

级别：详见MSDS文件

保质期：详见说明书

供应商：CST

库存：大量

适应物种：H,M,R,Mk,C

是否单克隆：2

保存条件：-20°c

规格：100 ul (10 western blots)/carrier free & custom formulation / quantity

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	H M R Mk (C)	Endogenous	78 to 82	Rabbit

Protocols	9454: Western Blotting
Specificity / Sensitivity	FoxO1 (L27) Antibody detects endogenous levels of total FoxO1 protein and does not cross-react with endogenous FoxO3a or FoxO4. The antibody also cross-reacts with an unidentified protein at 95 kDa.
Source / Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids within the amino terminus of mouse FoxO1. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting Western blot analysis of extracts from IGROV-1 cells and untreated, λ phosphatase- or FBS-treated (20 min.) HeLa cells using FoxO1 (L27) Antibody.
Background	The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). Anderson, M.J. et al. (1998) Genomics 47, 187-199. Galili, N. et al. (1993) Nat. Genet. 5, 230-235. Borkhardt, A. et al. (1997) Oncogene 14, 195-202. Nakae, J. et al. (1999) J. Biol. Chem. 274, 15982-15985. Rena, G. et al. (1999) J. Biol. Chem. 274, 17179-17183. Guo, S. et al. (1999) J. Biol. Chem. 274, 17184-17192. Seoane, J. et al. (2004) Cell 117, 211-223. Arden, K.C. (2004) Mol. Cell 14, 416-418. Yang, Y. et al. (2005) EMBO J. 24, 1021-1032.
Application References	Eke, I. et al. (2010) J Clin Invest 120, 2516-27. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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