Synapsin-1 (D12G5) XP® Rabbit mAb

价格：询价

品牌：Cell Signaling Technology 品牌认证

货号：5297

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抗体英文名：Synapsin-1 (D12G5) XP Rabbit mAb__®

抗原：synthetic peptide corresponding to residues surrounding Gln483 of human synapsin-1 protein

应用范围：W, IP, IHC-P, IF-F

库存：大量

供应商：CST

适应物种：H,M,R

保质期：详见说明书

级别：详见MSDS文件

是否单克隆：多克隆

保存条件：-20°c

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P IF-F	H M R	Endogenous	77	Rabbit IgG

Protocols	5297: IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity	Synapsin-1 (D12G5) XP^® Rabbit mAb detects endogenous levels of total synapsin protein. The antigen is 100% conserved between human synapsin-1a and synapsin-1b.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln483 of human synapsin-1 protein. Western Blotting Western blot analysis of extracts from CAD cells and neonatal mouse brain using Synapsin-1 (D12G5) XP^® Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human cerebellum using Synapsin-1 (D12G5) XP^® Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded mouse cortex using Synapsin-1 (D12G5) XP^® Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded rat retina using Synapsin-1 (D12G5) XP^® Rabbit mAb. IF-F Confocal immunofluorescent analysis of mouse brain using Synapsin-1 (D12G5) XP^® Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).
Background	Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2). Greengard, P. (1987) Mol Neurobiol 1, 81-119. Hosaka, M. et al. (1999) Neuron 24, 377-87.
Application References	Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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