BrdU (Bu20a) Mouse mAb

价格：询价

品牌：Cell Signaling Technology 品牌认证

货号：5292

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抗体英文名：BrdU (Bu20a) Mouse mAb

抗原：BrdU conjugated to BSA

应用范围：IHC-P, IF-IC, F

库存：大量

级别：详见MSDS文件

保质期：详见说明书

供应商：CST

适应物种：All

是否单克隆：1

保存条件：-20°c

规格：100 ul (100 assays)/carrier free & custom formulation / quantity

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Applications Key: IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Applications	Reactivity	Sensitivity	Isotype
IHC-P IF-IC F	All	Endogenous	Mouse IgG1

Protocols	5292: Flow, IHC / Paraffin, Immunofluorescence * Product-specific protocol.
Specificity / Sensitivity	BrdU (Bu20a) Mouse mAb detects BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody.
Source / Purification	Monoclonal antibody is produced by immunizing animals with BrdU conjugated to BSA. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded HeLa cells, without (left) and with (right) BrdU incorporation, using BrdU (Bu20a) Mouse mAb. Flow Cytometry Flow cytometric analysis of Jurkat cells incorporated with BrdU (30 minutes), using BrdU (Bu20a) Mouse mAb versus propidium iodide (DNA content). Flow Cytometry Flow cytometric analysis of Jurkat cells, untreated (blue) or BrdU incorporated for 30 minutes (green), using BrdU (Bu20a) Mouse mAb. IF-IC Confocal immunofluorescent analysis of HeLa cells using BrdU (Bu20a) Mouse mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).
Background	Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure as well as denaturation of double stranded DNA for various immunodetection applications (1-4). Darzynkiewicz, Z. and Juan, G. (2001) Curr Protoc Cytom Chapter 7, Unit 7.7. Leif, R.C. et al. (2004) Cytometry A 58, 45-52. Staszkiewicz, J. et al. (2009) Biochem Biophys Res Commun 378, 539-44. Rothaeusler, K. and Baumgarth, N. (2007) Curr Protoc Cytom Chapter 7, Unit7.31.
Application References	Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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