产品详情
文献和实验
相关推荐
抗体英文名 :14-3-3 ζ/δ (D7H5) Rabbit mAb
抗原 :synthetic peptide corresponding to residues surrounding Arg80 of human 14-3-3 ζ/δ protein
应用范围 :W
供应商 :CST
适应物种 :H,M,R,Mk,Pg,C
保质期 :详见说明书
库存 :大量
级别 :详见MSDS文件
是否单克隆 :1
保存条件 :-20°c
规格 :100 ul (10 western blots)/carrier free & custom formulation / quantity
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
---|---|---|---|---|
W | H M R Mk Pg (C) | Endogenous | 28 | Rabbit IgG |
Protocols |
|
---|---|
Specificity / Sensitivity | 14-3-3 ζ/δ (D7H5) Rabbit mAb recognizes endogenous levels of total 14-3-3 ζ/δ protein. Although this antibody demonstrates a strong preference for 14-3-3 ζ/δ, it will also detect purified, recombinant 14-3-3 α/β. It does not cross-react with any other known mammalian 14-3-3 isoforms. |
Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg80 of human 14-3-3 ζ/δ protein. |
Background | The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, has been observed (4). 14-3-3 binding masks specific sequences of the target protein and therefore modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3). |
Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know! |
Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
美国CST中国公司(丁香通商铺)
品牌商实名认证
钻石会员
入驻年限:11年