文献支持Mouse TNFRSF1A ELISA Pair Set | 小鼠 TNFRSF1A 酶联免疫配对抗体组合

保存条件 ：在-20℃ to -80℃

保质期 ：12个月

英文名 ：Mouse TNFRSF1A ELISA Pair Set

库存 ：99

供应商 ：北京义翘神州科技股份有限公司

规格 ：5 Plates/15 Plates

Mouse TNFRSF1A ELISA Pair Set（产品说明）
反应种属：Mouse
试剂对靶点：TNFRSF1A
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：4.69-300
试剂对标记类型：HRP
包被抗体：0.3 mg/mL of rabbit anti-Mouse TNFRSF1A monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50496-R104)
检测抗体：0.2 mg/mL of rabbit anti-Mouse TNFRSF1A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50496-R028)
试剂灵敏度：The minimum detectable dose of Mouse TNFRSF1A was determined to be approximately 4.69 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Mouse TNFRSF1A ELISA Pair Set is for the quantitative determination of Mouse TNFRSF1A.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Mouse TNFRSF1A coated on a 96-well plate. Standards and samples are added to the wells, and any Mouse TNFRSF1A present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Mouse TNFRSF1A monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Mouse TNFRSF1A present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：4.69 pg/ml