文献支持Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set | TNF-alpha / TNFA / TNFSF2
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保存条件 :在-20℃ to -80℃
保质期 :12个月
英文名 :Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set
库存 :99
供应商 :北京义翘神州科技股份有限公司
规格 :5 Plates/15 Plates
Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set ELISA Pair Set(产品说明)反应种属:Mouse
试剂对靶点:TNF
试剂对应用:ELISA
试剂对保存条件:Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围:
试剂对标记类型:HRP
包被抗体:1 mg/mL of rabbit anti-Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50349-R023)
检测抗体:0.2 mg/mL of rabbit anti-Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.2 μg/mL in Dilution Buffer before use.
试剂灵敏度:The minimum detectable dose of Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理:The Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set ELISA Pair Set is for the quantitative determination of Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set coated on a 96-well plate. Standards and samples are added to the wells, and any Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限:31.25 pg/ml
北京义翘神州科技股份有限公司(Sino Biological Inc.)
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入驻年限:15年