文献支持Human IL18RAP / IL1R7 ELISA Pair Set | 人 IL18RAP / IL1R7 酶联免疫配对抗体组合

保存条件 ：在-20℃ to -80℃

保质期 ：12个月

英文名 ：Human IL18RAP / IL1R7 ELISA Pair Set

库存 ：99

供应商 ：北京义翘神州科技股份有限公司

规格 ：5 Plates/15 Plates

Human IL18RAP / IL1R7 ELISA Pair Set（产品说明）
反应种属：Human
试剂对靶点：IL18RAP
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：7.81-500
试剂对标记类型：HRP
包被抗体：1 mg/mL of rabbit anti-Human IL18RAP / IL1R7 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10176-R033)
检测抗体：0.2 mg/mL of rabbit anti-Human IL18RAP / IL1R7 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10176-R122)
试剂灵敏度：The minimum detectable dose of Human IL18RAP / IL1R7 was determined to be approximately 7.81 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Human IL18RAP / IL1R7 ELISA Pair Set is for the quantitative determination of Human IL18RAP / IL1R7.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human IL18RAP / IL1R7 coated on a 96-well plate. Standards and samples are added to the wells, and any Human IL18RAP / IL1R7 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human IL18RAP / IL1R7 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human IL18RAP / IL1R7 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：7.81 pg/ml