文献支持Human MMP9 ELISA Pair Set

库存 ：99

供应商 ：北京义翘神州科技股份有限公司

检测限 ：125 pg/ml

检测方法 ：ELISA

应用 ：ELISA

适应物种 ：Human

标记物 ：HRP

规格 ：5 Plates/15 Plates

Human MMP9 ELISA Pair Set（产品说明）
反应种属：Human
试剂对靶点：MMP9
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：125-8000
试剂对标记类型：HRP
包被抗体：1 mg/mL of mouse anti-Human MMP9 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 10327-MM06)
检测抗体：0.2 mg/mL of rabbit anti-Human MMP9 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.1 μg/mL in Dilution Buffer before use. (Catalog: # 10327-R043)
试剂灵敏度：The minimum detectable dose of Human MMP9 was determined to be approximately 125 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Human MMP9 ELISA Pair Set is for the quantitative determination of Human MMP9. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human MMP9 coated on a 96-well plate. Standards and samples are added to the wells, and any Human MMP9 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human MMP9 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human MMP9 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：125 pg/ml