Human IL1R2 / IL1RB / CD121b ELISA Pair Set

库存 ：99

供应商 ：北京义翘神州科技股份有限公司

检测限 ：9.375 pg/ml

检测方法 ：ELISA

应用 ：ELISA

适应物种 ：Human

标记物 ：HRP

样本 ：见说明书

规格 ：5 Plates/15 Plates

Human IL1R2 / IL1RB / CD121b ELISA Pair Set（产品说明）
反应种属：Human
试剂对靶点：IL1R2
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：9.38-600
试剂对标记类型：HRP
包被抗体：0.5 mg/mL of rabbit anti-IL1R2 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating (Catalog: # 10111-R041)
检测抗体：0.5 mg/mL mouse anti-IL1R2 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10111-MM02)
试剂灵敏度：The minimum detectable dose of human IL1R2 (IL1RB / CD121b) was determined to be approximately 9.375 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard
试剂对检测原理：The human IL1R2 (IL1RB / CD121b) ELISA Pair Set is for the quantitative determinationof human IL1R2.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL1R2(IL1RB / CD121b) coated on a 96-well plate. Standards andsamples are added to the wells, and any IL1R2 ( IL1RB / CD121b) present binds tothe immobilized antibody. The wells are washed and a horseradish peroxidaseconjugated mouse anti-IL1R2 monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof IL1R2 present in the sample. To end the enzyme reaction, the stop solutionis added and absorbances of the microwell are read at 450 nm.
试剂对检测限：9.375 pg/ml