谷胱甘肽过氧化物酶分析试剂盒

产品名称：谷胱甘肽过氧化物酶试剂盒
英文名称：Glutathione Peroxidase Assay
货号：8238
品牌：ScienCell
产地：美国
规格：100 Tests

Description
Glutathione peroxidase (GPx) is an antioxidant enzyme located in cytoplasma and mitochondria. It catalyzes
the reduction of hydrogen peroxide (H2O2) and a variety of organic hydroperoxides (R-O-O-H) to water and the corresponding stable alcohols (R-OH) using glutathione as the reducing reagent, thus inhibiting the formation of free radicals and protecting
the cells from oxidative damage. The ScienCell GPx Assay provides an indirect method to quantify the
activity of total glutathione peroxidase. In this assay, oxidized glutathione, produced upon reduction of
peroxide substrate by glutathione peroxidase, is then recycled back to glutathione using glutathione
reductase and NADPH. The oxidation of NADPH to NADP+ is monitored by a decrease in the absorbance
at 340 nm. Since all other reagents are provided in excess, the GPx activity is rate-limiting. The rate of decrease in A340nm is directly proportional to the GPx activity. In this assay, cumene
hydroperoxide is used as the peroxide substrate, so the total GPx (selenium and non-selenium containing) activity in a variety of samples can be measured.


Product Use
This assay kit is used to evaluate glutathione peroxide activity in vitro. It is for research use only. Not for use
in animals, humans, or diagnostic procedures.