ScienCell 比色法GAPDH分析试剂盒

库存 ：大量

供应商 ：北京裕恒丰科技有限公司

英文名 ：GAPDH

规格 ：1000 tests/96 well plate

产品名称：比色法GAPDH 检测试剂盒
英文名称：Colorimetric GAPDH Assay
货号：8148
品牌：ScienCell
产地：美国
规格：100 tests

Description
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a tetrameric enzyme that catalyzes glycolysis and thus serves to
break down glucose for energy and carbon molecules. Since it is ubiquitously expressed at relatively
constant levels, GAPDH gene is widely used as an internal control for RNA and protein analysis. The
ScienCell? Colorimetric GAPDH Assay provides a nonradioactive method to measure the enzyme activity
of GAPDH in cultured cells, based on the oxidization of β-NADH to β-NAD in the presence of 3-phosphoglyceric acid (3-PGA), adenosine 5?-triphosphate (ATP) and GAPDH. The GAPDH activity is determined by assaying the rate of NADH oxidation,
which is proportional to the reduction in absorbance at 340 nm over time (δA340nm/min).


Product Use
This assay kit is used to evaluate GAPDH activity in vitro. It is for research use only. Not for use in animals,
humans, or diagnostic procedures.