文献支持Mouse IL-18 ELISA Kit（小鼠白介素18 ELISA试剂盒）

价格： ￥2650

品牌：MultiSciences 品牌认证

货号：70-EK218-96

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IL18 分子靶点信息概述

分子名：IL18, interleukin 18
基因家族：Interleukins
别名：IGIF; IL1F4; IL-1g; IL-18
全称：interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)

IL18 分子靶点综述

白细胞介素18(IL-18)，又名干扰素γ诱导因子，是一种促炎性细胞因子，在人类中由IL18基因编码。IL-18属于IL-1超家族，由巨噬细胞和其它细胞分泌产生。IL-18刺激后，自然杀伤细胞(NK)和某些T细胞就会释放另一种重要的细胞因子—干扰素γ或Ⅱ型干扰素，进而激活巨噬细胞或其它细胞。IL-18可诱导产生严重的炎症反应，同样可增加人类神经元中β-淀粉样蛋白(与阿尔茨海默氏病相关)的产生。

小鼠 Mouse Il18 分子靶点信息

分子名：Il18, interleukin 18
别称：
- Igif
- Il-18
- interferon gamma inducing factor
基因序列：NCBI_Gene: 16173
蛋白序列：
- UniprotKB: P70380
- UniprotKB: A0A1L1STF5
- UniprotKB: A0PJ18
- UniprotKB: K3W4N2
- UniprotKB: Q2PMY2
- UniprotKB: Q6USQ6
- UniprotKB: Q80SS8

小鼠 Mouse Il18 靶点分子功能（预测）

Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).

该产品被引用文献

IF = 34.915 Propionate and butyrate attenuate macrophage pyroptosis and osteoclastogenesis induced by CoCrMo alloy particles. Military Medical Research (2022)
IF = 15.881 Intravenous Delivery of Living Listeria monocytogenes Elicits Gasdmermin-Dependent Tumor Pyroptosis and Motivates Anti-Tumor Immune Response. ACS Nano (2022)
IF = 14.919 SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation. Nature Communications (2021)
IF = 14.143 Microneedle-assisted genome editing: A transdermal strategy of targeting NLRP3 by CRISPR-Cas9 for synergistic therapy of inflammatory skin disorders. Science Advances (2021)
IF = 13.391 USP22 suppresses the NLRP3 inflammasome by degrading NLRP3 via ATG5-dependent autophagy. Autophagy (2022)
IF = 12.416 Mitochondrial calpain-1 activates NLRP3 inflammasome by cleaving ATP5A1 and inducing mitochondrial ROS in CVB3-induced myocarditis. BASIC RESEARCH IN CARDIOLOGY (2022)
IF = 10.787 ChemR23 signaling ameliorates cognitive impairments in diabetic mice via dampening oxidative stress and NLRP3 inflammasome activation. Redox Biology (2022)
IF = 10.435 The ketone body β-hydroxybutyrate alleviates CoCrMo alloy particles induced osteolysis by regulating NLRP3 inflammasome and osteoclast differentiation. JOURNAL OF NANOBIOTECHNOLOGY (2022)
IF = 9.995 Gasdermin D restricts anti-tumor immunity during PD-L1 checkpoint blockade. Cell Reports (2022)
IF = 9.986 NLRP3 inflammasome-mediated pyroptosis contributes to the pathogenesis of non-ischemic dilated cardiomyopathy. Redox Biology (2020)
IF = 9.587 Directly targeting ASC by lonidamine alleviates inflammasome-driven diseases. Journal of Neuroinflammation (2022)
IF = 8.786 GSDMD contributes to myocardial reperfusion injury by regulating pyroptosis. Frontiers in Immunology (2022)
IF = 8.786 Munronoid I Ameliorates DSS-Induced Mouse Colitis by Inhibiting NLRP3 Inflammasome Activation and Pyroptosis Via Modulation of NLRP3. Frontiers in Immunology (2022)
IF = 8.755 Tetrahedral framework nucleic acid nanomaterials reduce the inflammatory damage in sepsis by inhibiting pyroptosis. CELL PROLIFERATION (2023)
IF = 8.679 Arctigenin disrupts NLRP3 inflammasome assembly in colonic macrophages via downregulating fatty acid oxidation to prevent colitis-associated cancer. CANCER LETTERS (2020)
IF = 8.579 Stem cell-derived exosomes prevent pyroptosis and repair ischemic muscle injury through a novel exosome/circHIPK3/ FOXO3a pathway. Theranostics (2020)
IF = 8.469 A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis. Cell Death & Disease (2021)
IF = 8.469 DHA/AA alleviates LPS-induced Kupffer cells pyroptosis via GPR120 interaction with NLRP3 to inhibit inflammasome complexes assembly. Cell Death & Disease (2021)
IF = 7.675 Bisdemethoxycurcumin Alleviates Dextran Sodium Sulfate-induced Colitis via Inhibiting NLRP3 Inflammasome Activation and Modulating the Gut Microbiota in Mice. Antioxidants (2022)
IF = 7.666 HHcy Induces Pyroptosis and Atherosclerosis via the Lipid Raft-Mediated NOX-ROS-NLRP3 Inflammasome Pathway in apoE−/− Mice. Cells (2022)
IF = 7.59 Quercetin alleviates nanoparticles induced osteolysis via deactivating pyroptosis. Biomaterials Science (2023)
IF = 7.561 NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection. Frontiers in Immunology (2021)
IF = 7.525 Mesenchymal stem cells protect against TBI-induced pyroptosis in vivo and in vitro through TSG-6. Cell Communication and Signaling (2022)
IF = 7.233 Mesoporous silica nanoparticles induced hepatotoxicity via NLRP3 inflammasome activation and caspase-1-dependent pyroptosis. Nanoscale (2018)
IF = 7.129 YAP1 protects against PM2.5-induced lung toxicity by suppressing pyroptosis and ferroptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023)
IF = 7.086 Activation of pyroptosis and ferroptosis is involved in the hepatotoxicity induced by polystyrene microplastics in mice. CHEMOSPHERE (2021)
IF = 6.922 Resveratrol Relieves Gouty Arthritis by Promoting Mitophagy to Inhibit Activation of NLRP3 Inflammasomes. Journal of Inflammation Research (2021)
IF = 6.684 miR-513c-5p Suppression Aggravates Pyroptosis of Endothelial Cell in Deep Venous Thrombosis by Promoting Caspase-1. Frontiers in Cell and Developmental Biology (2022)
IF = 6.543 TSPO Ligands Protect against Neuronal Damage Mediated by LPS-Induced BV-2 Microglia Activation. Oxidative Medicine and Cellular Longevity (2022)
IF = 6.53 Astragaloside IV alleviates PM2.5-caused lung toxicity by inhibiting inflammasome‐mediated pyroptosis via NLRP3/caspase-1 axis inhibition in mice. BIOMEDICINE & PHARMACOTHERAPY (2022)
IF = 6.53 Knockdown of TLR4 attenuates high glucose-induced podocyte injury via the NALP3/ASC/Caspase-1 signaling pathway. BIOMEDICINE & PHARMACOTHERAPY (2018)
IF = 6.53 Natural molecule Munronoid I attenuates LPS-induced acute lung injury by promoting the K48-linked ubiquitination and degradation of TAK1. BIOMEDICINE & PHARMACOTHERAPY (2021)
IF = 6.53 The Chinese herbal medicine Fufang Zhenzhu Tiaozhi protects against diabetic cardiomyopathy by alleviating cardiac lipotoxicity-induced oxidative stress and NLRP3-dependent inflammasome activation. BIOMEDICINE & PHARMACOTHERAPY (2022)
IF = 6.388 Berberine plays a cardioprotective role by inhibiting macrophage Wnt5a/β-catenin pathway in the myocardium of mice after myocardial infarction. PHYTOTHERAPY RESEARCH (2022)
IF = 6.183 AMSC-derived exosomes alleviate lipopolysaccharide/d-galactosamine-induced acute liver failure by miR-17-mediated reduction of TXNIP/NLRP3 inflammasome activation in macrophages. EBioMedicine (2018)
IF = 6.17 Baicalin promotes liver regeneration after acetaminophen-induced liver injury by inducing NLRP3 inflammasome activation. FREE RADICAL BIOLOGY AND MEDICINE (2020)
IF = 6.17 Oroxylin a promotes PGC-1α/Mfn2 signaling to attenuate hepatocyte pyroptosis via blocking mitochondrial ROS in alcoholic liver disease. FREE RADICAL BIOLOGY AND MEDICINE (2020)
IF = 6.124 AIM2 inflammasome contributes to aldosterone-induced renal injury via endoplasmic reticulum stress. CLINICAL SCIENCE (2022)
IF = 5.959 miR-21 promotes NLRP3 inflammasome activation to mediate pyroptosis and endotoxic shock. Cell Death & Disease (2019)
IF = 5.924 Single-Chain Fragment Variables Targeting Leukocidin ED Can Alleviate the Inflammation of Staphylococcus aureus-Induced Mastitis in Mice. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)
IF = 5.895 T-2 Toxin Caused Mice Testicular Inflammation Injury via ROS-Mediated NLRP3 Inflammasome Activation. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)
IF = 5.858 Identification of Tubocapsanolide A as a novel NLRP3 inhibitor for potential treatment of colitis. BIOCHEMICAL PHARMACOLOGY (2021)
IF = 5.811 Micronized Palmitoylethanolamide Ameliorates Methionine- and Choline-Deficient Diet-Induced Nonalcoholic Steatohepatitis via Inhibiting Inflammation and Restoring Autophagy. Frontiers in Pharmacology (2021)
IF = 5.793 Sodium para-aminosalicylic acid inhibits manganese-induced NLRP3 inflammasome-dependent pyroptosis by inhibiting NF-κB pathway activation and oxidative stress. Journal of Neuroinflammation (2020)
IF = 5.714 4-octyl itaconate ameliorates alveolar macrophage pyroptosis against ARDS via rescuing mitochondrial dysfunction and suppressing the cGAS/STING pathway. INTERNATIONAL IMMUNOPHARMACOLOGY (2023)
IF = 5.714 Fibroblast growth factor 10 protects against particulate matter-induced lung injury by inhibiting oxidative stress-mediated pyroptosis via the PI3K/Akt/Nrf2 signaling pathway. INTERNATIONAL IMMUNOPHARMACOLOGY (2022)
IF = 5.714 Hyperhomocysteinemia activates NLRP3 inflammasome to cause hepatic steatosis and insulin resistance via MDM2-mediated ubiquitination of HSF1. INTERNATIONAL IMMUNOPHARMACOLOGY (2023)
IF = 5.7 Peptide MSI-1 inhibited MCR-1 and regulated outer membrane vesicles to combat immune evasion of Escherichia coli. Microbial Biotechnology (2023)
IF = 5.6 LMCD1 is involved in tubulointerstitial inflammation in the early phase of renal fibrosis by promoting NFATc1-mediated NLRP3 activation. INTERNATIONAL IMMUNOPHARMACOLOGY (2023)
IF = 5.572 Salidroside pretreatment alleviates PM2.5 caused lung injury via inhibition of apoptosis and pyroptosis through regulating NLRP3 Inflammasome. FOOD AND CHEMICAL TOXICOLOGY (2023)
IF = 5.511 NOD-Like Receptor Protein 3 Inflammasome-Dependent IL-1β Accelerated ConA-Induced Hepatitis. Frontiers in Immunology (2018)
IF = 5.4 Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2023)
IF = 5.344 Cytokine-induced killer cells-assisted tumor-targeting delivery of Her-2 monoclonal antibody-conjugated gold nanostars with NIR photosensitizer for enhanced therapy of cancer. Journal of Materials Chemistry B (2020)
IF = 5.314 Irisin attenuates acute lung injury by suppressing the pyroptosis of alveolar macrophages. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2023)
IF = 5.241 Mitochondrial damage and activation of the cytosolic DNA sensor cGAS–STING pathway lead to cardiac pyroptosis and hypertrophy in diabetic cardiomyopathy mice. Cell Death Discovery (2022)
IF = 5.223 6-Gingerol attenuates arsenic trioxide-induced liver injury by inhibiting pyroptosis and ROS-NLRP3 inflammatory signaling pathway: Based on network pharmacology analysis and experiment verification. Journal of Functional Foods (2023)
IF = 5.195 Polydatin protects against atherosclerosis by activating autophagy and inhibiting pyroptosis mediated by the NLRP3 inflammasome. JOURNAL OF ETHNOPHARMACOLOGY (2023)
IF = 5.135 HOTTIP downregulation reduces neuronal damage and microglial activation in Parkinson's disease cell and mouse models. Neural Regeneration Research (2021)
IF = 5.097 Novel biphenyl diester derivative AB-38b inhibits NLRP3 inflammasome through Nrf2 activation in diabetic nephropathy. CELL BIOLOGY AND TOXICOLOGY (2019)
IF = 5.073 Gasdermin D–mediated pyroptosis suppresses liver regeneration after 70% partial hepatectomy. Hepatology Communications (2022)
IF = 5.037 High-mobility group box 1 protein participates in acute lung injury by activating protein kinase R and inducing M1 polarization. LIFE SCIENCES (2020)
IF = 4.932 Loganin alleviates sepsis-induced acute lung injury by regulating macrophage polarization and inhibiting NLRP3 inflammasome activation. INTERNATIONAL IMMUNOPHARMACOLOGY (2021)
IF = 4.932 Protective effects and possible mechanism of 6-gingerol against arsenic trioxide-induced nephrotoxicity based on network pharmacological analysis and experimental validation. INTERNATIONAL IMMUNOPHARMACOLOGY (2022)
IF = 4.927 Pine Pollen Polysaccharides’ and Sulfated Polysaccharides’ Effects on UC Mice through Modulation of Cell Tight Junctions and RIPK3-Dependent Necroptosis Pathways. MOLECULES (2022)
IF = 4.926 Mycobacterium tuberculosis PE_PGRS19 Induces Pyroptosis through a Non-Classical Caspase-11/GSDMD Pathway in Macrophages. Microorganisms (2022)
IF = 4.925 Aim2 Deficiency Ameliorates Lacrimal Gland Destruction and Corneal Epithelium Defects in an Experimental Dry Eye Model. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2023)
IF = 4.9 Du Huo Ji Sheng Tang inhibits Notch1 signaling and subsequent NLRP3 activation to alleviate cartilage degradation in KOA mice. Chinese Medicine (2023)
IF = 4.716 Interleukin-22 Attenuated Renal Tubular Injury in Aristolochic Acid Nephropathy via Suppressing Activation of NLRP3 Inflammasome. Frontiers in Immunology (2019)
IF = 4.663 A Neonatal Murine Model of Coxsackievirus A6 Infection for Evaluation of Antiviral and Vaccine Efficacy | Journal of Virology. JOURNAL OF VIROLOGY (2017)
IF = 4.663 Protective Efficacies of Formaldehyde-Inactivated Whole-Virus Vaccine and Antivirals in a Murine Model of Coxsackievirus A10 Infection | Journal of Virology. JOURNAL OF VIROLOGY (2017)
IF = 4.565 miR-146a is directly regulated by STAT3 in human hepatocellular carcinoma cells and involved in anti-tumor immune suppression. CELL CYCLE (2015)
IF = 4.56 Murine model of acute myocarditis and cerebral cortical neuron edema induced by coxsackievirus B4. ZOOLOGICAL RESEARCH (2018)
IF = 4.529 Human Neutrophil Defensins Disrupt Liver Interendothelial Junctions and Aggravate Sepsis. MEDIATORS OF INFLAMMATION (2022)
IF = 4.5 Upregulation of postsynaptic cAMP/PKA/CREB signaling alleviates copper(Ⅱ)-induced oxidative stress and pyroptosis in MN9D cells. TOXICOLOGY (2023)
IF = 4.451 Neuroprotective effects of natural cordycepin on LPS-induced Parkinson’s disease through suppressing TLR4/NF-κB/NLRP3-mediated pyroptosis. Journal of Functional Foods (2020)
IF = 4.422 Anti-Staphylococcus aureus Single-Chain Fragment Variables Play a Protective Anti-Inflammatory Role In Vitro and In Vivo. Vaccines (2021)
IF = 4.412 Characterization, Antioxidant and Anti-Inflammation Capacities of Fermented Flammulina velutipes Polyphenols. MOLECULES (2021)
IF = 4.369 Upregulation of TXNIP contributes to granulosa cell dysfunction in polycystic ovary syndrome via activation of the NLRP3 inflammasome. MOLECULAR AND CELLULAR ENDOCRINOLOGY (2022)
IF = 4.315 Beclin-1 overexpression regulates NLRP3 activation by promoting TNFAIP3 in microvascular injury following myocardial reperfusion. CELLULAR SIGNALLING (2021)
IF = 4.225 Evodiamine Attenuates Experimental Colitis Injury Via Activating Autophagy and Inhibiting NLRP3 Inflammasome Assembly. Frontiers in Pharmacology (2020)
IF = 4.225 Rhein Suppresses Lung Inflammatory Injury Induced by Human Respiratory Syncytial Virus Through Inhibiting NLRP3 Inflammasome Activation via NF-κB Pathway in Mice. Frontiers in Pharmacology (2020)
IF = 4.101 HMGB1 participates in LPS‑induced acute lung injury by activating the AIM2 inflammasome in macrophages and inducing polarization of M1 macrophages via TLR2, TLR4, and RAGE/NF‑κB signaling pathways Corrigendum in /10.3892/ijmm.2020.4530. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019)
IF = 3.996 Liraglutide Ameliorates Cerebral Ischemia in Mice via Antipyroptotic Pathways. NEUROCHEMICAL RESEARCH (2022)
IF = 3.943 Libertellenone M, a diterpene derived from an endophytic fungus Phomopsis sp. S12, protects against DSS-induced colitis via inhibiting both nuclear translocation of NF-κB and NLRP3 inflammasome activation. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
IF = 3.919 Targeting HDAC6 attenuates nicotine-induced macrophage pyroptosis via NF-κB/NLRP3 pathway. ATHEROSCLEROSIS (2020)
IF = 3.876 Role of ASM/Cer/TXNIP signaling module in the NLRP3 inflammasome activation. Lipids in Health and Disease (2021)
IF = 3.829 Down-regulation of long non-coding RNA SNHG14 protects against acute lung injury induced by lipopolysaccharide through microRNA-34c-3p-dependent inhibition of WISP1. RESPIRATORY RESEARCH (2019)
IF = 3.758 Monocytes Undergo Functional Reprogramming to Generate Immunosuppression through HIF-1α Signaling Pathway in the Late Phase of Sepsis. MEDIATORS OF INFLAMMATION (2020)
IF = 3.715 Mild hypothermia alleviates early brain injury after subarachnoid hemorrhage via suppressing pyroptosis through AMPK/NLRP3 inflammasome pathway in rats. BRAIN RESEARCH BULLETIN (2022)
IF = 3.628 Obeticholic acid improved triptolide/lipopolysaccharide-induced hepatotoxicity by inhibiting Caspase-11-GSDMD pyroptosis pathway. JOURNAL OF APPLIED TOXICOLOGY (2022)
IF = 3.457 Arctigenin protects against liver injury from acute hepatitis by suppressing immune cells in mice. BIOMEDICINE & PHARMACOTHERAPY (2018)
IF = 3.361 Pre-activation of TLR3 enhances the therapeutic effect of BMMSCs through regulation the intestinal HIF-2α signaling pathway and balance of NKB cells in experimental alcoholic liver injury. INTERNATIONAL IMMUNOPHARMACOLOGY (2019)
IF = 3.361 Protective effect of Ketone musk on LPS/ATP-induced pyroptosis in J774A.1 cells through suppressing NLRP3/GSDMD pathway. INTERNATIONAL IMMUNOPHARMACOLOGY (2019)
IF = 3.244 Circadian rhythm disorders elevate macrophages cytokines release and promote multiple tissues/organs dysfunction in mice. PHYSIOLOGY & BEHAVIOR (2022)
IF = 3.044 NLRC3 Participates in Inhibiting the Pulmonary Inflammatory Response of Sepsis-Induced Acute Lung Injury. IMMUNOLOGICAL INVESTIGATIONS (2023)
IF = 2.952 Resveratrol affects the expression of uric acid transporter by improving inflammation. Molecular Medicine Reports (2021)
IF = 2.86 Murine Cytomegalovirus Infection Induced miR-1929-3p Down-Regulation Promotes the Proliferation and Apoptosis of Vascular Smooth Muscle Cells in Mice by Targeting Endothelin A Receptor and Downstream NLRP3 Activation Pathway. MOLECULAR BIOTECHNOLOGY (2023)
IF = 2.822 Artesunate Protects Pancreatic β-Cells From Streptozotocin-Induced Diabetes via Inhibition of the NLRP3/Caspase-1/GSDMD Pathway. GENERAL AND COMPARATIVE ENDOCRINOLOGY (2022)
IF = 2.742 BRD4 inhibition by JQ1 protects against LPS-induced cardiac dysfunction by inhibiting activation of NLRP3 inflammasomes. MOLECULAR BIOLOGY REPORTS (2022)
IF = 2.723 Inhibition of perilipin 2 attenuates cerebral ischemia/reperfusion injury by blocking NLRP3 inflammasome activation both in vivo and in vitro. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2023)
IF = 2.68 Dimethyl fumarate ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis through enhancing Nrf2 signaling. Toxicology Research (2022)
IF = 2.65 Prevention of Bleomycin-Induced Pulmonary Inflammation and Fibrosis in Mice by Bilobalide. Evidence-based Complementary and Alternative Medicine (2023)
IF = 2.63 LuQi Formula Ameliorates Myocardial Fibrosis by Suppressing TLR4/MyD88/NF-κB Pathway and NLRP3 Inflammasome Activation in Mice with Myocardial Infarction. Evidence-based Complementary and Alternative Medicine (2022)
IF = 2.6 Huoxue Jiedu Recipe represses mitochondrial fission to alleviate submandibular gland inflammation in Sjögren's syndrome. MICROBIOLOGY AND IMMUNOLOGY (2023)
IF = 2.447 P2X7R antagonist protects against renal injury in mice with adriamycin nephropathy. Experimental and Therapeutic Medicine (2021)
IF = 2.349 6-Shogaol alleviates CCl4-induced liver fibrosis by attenuating inflammatory response in mice through the NF-κB pathway. ACTA BIOCHIMICA POLONICA (2022)
IF = 2.264 Tilianin Protects against Nonalcoholic Fatty Liver Disease in Early Obesity Mice. BIOLOGICAL & PHARMACEUTICAL BULLETIN (2023)
IF = 2.086 Kaempferol alleviates LPS-ATP mediated inflammatory injury in splenic lymphocytes via regulation of the pyroptosis pathway in mice. IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2019)
IF = 0 NOD1 induces pyroptotic cell death to aggravate liver ischemia-reperfusion injury in mice. MedComm (2022)

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背景介绍	白细胞介素18(IL-18)，又名干扰素γ诱导因子，是一种促炎性细胞因子，在人类中由IL18基因编码。IL-18属于IL-1超家族，由巨噬细胞和其它细胞分泌产生。 IL-18刺激后，自然杀伤细胞(NK)和某些T细胞就会释放另一种重要的细胞因子—干扰素γ或Ⅱ型干扰素，进而激活巨噬细胞或其它细胞。IL-18可诱导产生严重的炎症反应，同样可增加人类神经元中β-淀粉样蛋白(与阿尔茨海默氏病相关)的产生。
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-18抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的IL-18与固相抗体结合和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-18的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
组分	包被抗小鼠IL-18单克隆抗体的96孔聚苯乙烯酶标板小鼠IL-18 冻干标准品 IL-18 检测抗体辣根过氧化物酶标记的链霉亲和素检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜
分析类型	夹心ELISA
样本类型	血清、血浆，细胞培养上清及其他生物学样本
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
样本体积	20 μl
灵敏度	0.93 pg/ml
标曲图
标准曲线范围	62.4pg/ml - 4000pg/ml
回收率范围	80% - 119 %
平均回收率	0.98
板间变异系数	2.7% - 4.0%
板内变异系数	2.4% -3.7%