免疫原 ：hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)

亚型 ：IgG1

形态 ：liquid

克隆性 ：单克隆

适应物种 ：Human

保质期 ：12 month

库存 ：50

供应商 ：LSM bio

宿主 ：Mouse

应用范围 ：ELISA, IF, WB

浓度 ：≥95% as determined by SDS-PAGE

抗体英文名 ：HIF1a antibody

抗体名 ：HIF1a antibody

规格 ：100ul

HIF1a antibody

Product Name HIF1a antibody

Catalog No PAab09940

Packing 100ul

Form liquid

Alternative Name BHLHE78, MOP1, PASD8

Purification protein A+G purified

Purity 95% as determined by SDS-PAGE

Host Mouse

Isotype IgG1

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918).

Immunogen hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)

Specificity Human

Tested Application ELISA, IF, WB

Recommended dilution WB: 1:1000-1:10000; IF: 1:50-1:200

IHC use

WB use

Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with PAab09940(HIF1a Antibody) at dilution of 1:5000

Protein Information

Gene ID 3091

Uniprot ID Q16665

Calculated MW 120 kDa

Research Area Signal Transduction, Metabolism, Cardiovascular, Developmental biology, Neuroscience, Stem Cells