Bovine Spindle and kinetochore- associated protein 2, SKA2 ELISA

库存 ：货源充足

供应商 ：LSM Bio

检测限 ：详见说明书

检测方法 ： 夹心法/竞争法

应用 ：夹心法/竞争法ELISA体外定量检测小鼠血清、血浆或其它相关生物液体中蛋白浓度

适应物种 ：牛

标记物 ：详见说明书

样本 ：血清、血浆或其它相关生物液体中天然和重组蛋白

规格 ：96T

Bovine Spindle and kinetochore- associated protein 2, SKA2 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

SKA2,FAM33A; family with sequence similarity 33, member A; spindle and KT (kinetochore) associated 2; spindle and kinetochore-associated protein 2; spindle and kinetochore associated complex subunit 2

 

Search name

Bovine SKA2 ELISA KIT ,Bovine FAM33A ELISA KIT ,Bovine family with sequence similarity 33 member A ELISA KIT ,Bovine spindle and KT (kinetochore) associated 2 ELISA KIT ,Bovine spindle and kinetochore-associated protein 2 ELISA KIT ,Bovine spindle and kinetochore associated complex subunit 2 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Spindle and kinetochore- associated protein 2, SKA2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SKA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SKA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SKA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SKA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

 

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