Bovine Glucagon, GCG ELISA KIT

库存 ：货源充足

供应商 ：LSM Bio

检测限 ：详见说明书

检测方法 ： 夹心法/竞争法

应用 ：夹心法/竞争法ELISA体外定量检测小鼠血清、血浆或其它相关生物液体中蛋白浓度

适应物种 ：牛

标记物 ：详见说明书

样本 ：血清、血浆或其它相关生物液体中天然和重组蛋白

规格 ：96T

Bovine Glucagon, GCG ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Glucagon,glucagon-like peptide 2; glucagon-like peptide 1; GLP1; GLP2; GRPP; glicentin-related polypeptide,GcG, preproglucagon

Search name
Bovine Glucagon ELISA Kit, Bovine glucagon-like peptide 2 ELISA Kit, Bovine glucagon-like peptide 1 ELISA Kit, Bovine GLP1
ELISA Kit, Bovine GLP2 ELISA Kit, Bovine GRPP ELISA Kit, Bovine GC ELISA Kit, Bovine glicentin-related polypeptide ELISA
Kit, Bovine preproglucagon ELISA KIT,Bovine GCG ELISA KIT

Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine glucagon,Glucagon concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucagon. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glucagon and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Glucagon, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glucagon in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

PDF manual download

 

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