磷酸化染色体浓缩调控蛋白1抗体价格

供应商：江西江蓝纯生物试剂有限公司

库存：22

克隆性：单克隆

保质期：1年

抗体英文名：Phospho-RCC1 (Ser11)

抗体名：磷酸化染色体浓缩调控蛋白1抗体

适应物种：人/动物/植物

应用范围：WB,ELISA等

浓度：1mg/ml

保存条件：-20 °

规格：100ul

产品货号 :  jlcR3360
英文名称 :  Phospho-RCC1 (Ser11)
中文名称 : 磷酸化染色体浓缩调控蛋白1抗体
别    名 : Cell cycle regulatory protein; CHC 1; CHC1; Chromosome condensation 1; Chromosome condensation protein 1; Guanine nucleotide releasing protein; HERC2; Ran GEF; RanGEF; RCC 1; RCC1 I; Regulator of chromosome condensation 1; Regulator of chromosome condensation; SHEP1; RCC1_HUMAN.
产品类型 : 磷酸化抗体
研究领域 : 细胞生物染色质和核信号信号转导细胞凋亡转录调节因子激酶和磷酸酶
抗体来源 : Rabbit
克隆类型 : Polyclonal
交叉反应 :   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
产品应用 : ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分子量 : 45kDa
细胞定位 : 细胞核细胞浆
性    状 : Lyophilized or Liquid
浓    度 : 1mg/ml
免疫原 : KLH conjugated Synthesised phosphopeptide derived from human RCC1 around the phosphorylation site of Ser11:RR(p-S)PP
亚    型 : IgG
纯化方法 : affinity purified by Protein A
储存液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 : Ran GTPase plays important roles in nucleocytoplasmic transport in interphase and in both spindle formation and nuclear envelope (NE) assembly during mitosis. The latter functions rely on the presence of high local concentrations of GTP bound Ran near mitotic chromatin. RanGTP localization has been proposed to result from the association of Ran's GDP/GTP exchange factor, RCC1, with chromatin , but Ran is shown here to bind directly to chromatin in two modes, either dependent or independent of RCC1, and, where bound, to increase the affinity of chromatin for NE membranes.

Function:
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.

Subunit:
Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.

Subcellular Location:
Nucleus. Cytoplasm. Note=Becomes dispersed throughout the cytoplasm during mitosis.

Post-translational modifications:
N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.

Similarity:
Contains 7 RCC1 repeats.

SWISS:
P18754

Gene ID:
1104

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.