Complement C4-B, C4b ELISA KIT/ Mouse Complement C4-B, C4b ELISA试剂盒

价格： ￥2980

品牌：LSM Bio

货号：ELI-33888m

产品详情

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库存：货源充足

供应商：LSM Bio

检测限：详见说明书

检测方法：夹心法ELISA或竞争法ELISA

应用：详见说明书

适应物种：小鼠

标记物：目的小分子或该目的蛋白

样本：小鼠血清，血浆，组织匀浆

规格：96T

Mouse Complement C4 -B, C4B ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Complement C4-B,Basic complement C4,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3,

Search name

Mouse C4B ELISA KIT ,Mouse C4B1 ELISA KIT ,Mouse C4B12 ELISA KIT ,Mouse C4B2 ELISA KIT ,Mouse C4B3 ELISA KIT ,Mouse C4B5 ELISA KIT ,Mouse C4BD ELISA KIT ,Mouse C4B_2 ELISA KIT ,Mouse C4F ELISA KIT ,Mouse CO4 ELISA KIT ,Mouse CPAMD3 ELISA KIT ,Mouse C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA KIT ,Mouse Chido form of C4 ELISA KIT ,Mouse basic complement C4 ELISA KIT ,Mouse complement C4B1a ELISA KIT ,Mouse complement component 4B ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse Complement C4-B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement C4-B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Complement C4-B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Complement C4-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Complement C4-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.