Triiodothyronine, T3 ELISA Kit/ Mouse Triiodothyronine, T3 ELISA试剂盒

价格： ￥2980

品牌：LSM Bio

货号：CELI-66097m

产品详情

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库存：货源充足

供应商：LSM Bio

检测限：详见说明书

检测方法：夹心法ELISA或竞争法ELISA

应用：详见说明书

适应物种：小鼠

标记物：目的小分子或该目的蛋白

样本：小鼠血清，血浆，组织匀浆

规格：96T

Mouse Triiodothyronine, T3 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Triiodothyronine,T3, 3,3',5-triiodo-L-thyronine

Search name

Mouse T3 ELISA Kit, Mouse Triiodothyronine ELISA Kit, Mouse 3,3',5-triiodo-L-thyronine ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Triiodothyronine concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Triiodothyronine, During the reaction, Triiodothyronine in the sample or standard competes with a fixed amount of biotin-labeled Triiodothyronine for sites on a pre-coated Monoclonal antibody specific to Triiodothyronine. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Triiodothyronine in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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